The specific dye is considered proprietary. However, the red dye used to prepare this product has an excitation wavelength of 575 nm and emission wavelength of 610 nm.
L3280
Latex beads, carboxylate-modified polystyrene, fluorescent red
aqueous suspension, 0.5 μm mean particle size
Synonym(s):
Carboxylate Polystyrene Beads, Fluorescent Latex Beads
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Available to ship TODAYfromMILWAUKEE | $73.30 |
About This Item
$73.30
Available to ship TODAYDetails
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form
aqueous suspension
Quality Segment
composition
Solids, 2.5%
technique(s)
cell based assay: suitable
mean particle size
0.5 μm
fluorescence
λex ~575 nm; λem ~610 nm
application(s)
cell analysis
Application
Latex beads, carboxylate-modified polystyrene, fluorescent red has been used:
- along with Milli-Q for fluid dynamic studies
- along with bile salts for confocal studies
- for particle encapsulation
Carboxylate-modified polystyrene latex beads have been used to collect morphofunctional data about the immune systems of Carabus lefebvrei, a helicophagous Italian endemic ground beetle often used as an indicator of the habitat quality of the Apennines mountain forests. Carboxylate-modified polystyrene latex beads have also been used to inform comparative studies on immunity defense mechanisms of adults and larvae of the coleopteran Cetonischema aeruginosa and to investigate the phagocytic activity of cultured rat thyroid cells.
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This Item | |||
|---|---|---|---|
| description aqueous suspension, 0.5 μm mean particle size | description aqueous suspension, 2.0 μm mean particle size | description aqueous suspension, 1.0 μm mean particle size | description aqueous suspension, 0.5 μm mean particle size |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) - |
| fluorescence λex ~575 nm; λem ~610 nm | fluorescence λex ~553 nm; λem ~635 nm | fluorescence λex ~575 nm; λem ~610 nm | fluorescence λex ~520 nm; λem ~540 nm |
| form aqueous suspension | form aqueous suspension | form aqueous suspension | form aqueous suspension |
| application(s) cell analysis | application(s) cell analysis | application(s) cell analysis | application(s) - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| composition Solids, 2.5% | composition Solids, 2.5% | composition Solids, 2.5% | composition Solids, 2.5% |
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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What is the name of the fluorescent red dye used in these beads?
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Is this product supplied sterile? If not, do you have a recommended sterilization protocol for the microplastics, such as ethanol treatment, gamma irradiation, or ethylene oxide?
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This product is not supplied sterile. No validated sterilization protocol exists specifically for this product. Polystyrene latex/microparticles can be sterilized by several methods. Autoclaving is not recommended because polystyrene may soften at autoclave temperatures, potentially causing aggregation or changes to particle characteristics. This product has not been evaluated for the presence or removal of microplastics.
Possible approaches to consider (not validated for this product):
70% Ethanol treatment: Incubate in 70% ethanol for ~30 minutes, then rinse thoroughly with sterile water or sterile buffer to remove residual ethanol.
UV irradiation: Suitable for very dilute suspensions where UV light can penetrate the sample.
Pasteurization: Heat bead suspension at 70–80°C for 24 hours to reduce microbial contamination.
Gamma irradiation: Reported as a potential sterilization method for latex bead preparations.
Antimicrobial preservatives: Agents such as sodium azide, thimerosal, or formaldehyde may inhibit microbial growth where appropriate for the intended application.
Note: These methods have not been specifically validated for this product. Conduct a small-scale evaluation to confirm sterilization method compatibility with the intended application. For reference, see the following technical documents on latex bead handling and sterilization:https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/348/819/lb1pis-ms.pdf
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/166/102/l0655pis.pdf
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How many latex beads (500 nm) are present in 1 mL solution?
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Unfortunately, the bead count for this product has not been determined. The particle size is approximately 0.5 um and the solid content is approximately 2.5%. Please see the link below to review the full product specifications:
https://www.sigmaaldrich.com/specification-sheets/357/795/L3280-BULK________SIGMA____.pdfHelpful?
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How many fluorescent beads (L3280) are in the 1 ml of the product?
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The bead count for this product is not a tested specification. As listed in the "Properties" section of the product detail page, the solids constitute 2.5% of the aqueous solution. This specification can also be viewed on the sample Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/L3280-BULKMKCS6365.pdf.
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