Works excellent in the animal model.................
L3755
Lipopolysaccharides from Escherichia coli O26:B6
purified by trichloroacetic acid extraction
Lipopolysaccharides from Escherichia coli O26:B6
Synonym(s):
LPS
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| Size/SKU | Availability | Price |
|---|---|---|
100 mg | Please contact Customer Service for Availability | R 4 476,00 R 4 386,48 |
500 mg | Please contact Customer Service for Availability | R 16 646,00 R 16 313,08 |
About This Item
R 4 386,48
List PriceR 4 476,00Save 2%Please contact Customer Service for Availability
biological source
Escherichia coli (O26:B6)
Quality Segment
form
lyophilized powder
purified by
trichloroacetic acid extraction
impurities
1-10% Protein (Lowry)
color
white to yellow
solubility
water: soluble
shipped in
ambient
storage temp.
2-8°C
General description
This product is TCA extracted from E. coli serotype O26:B6. The source strain is ATCC 12795. LPS from serotype 026:B6 is the only LPS from E. coli which exhibits short chain-length behavior on SDS-PAGE. The short chain-length of this LPS is closer to that of the mutant rough strain LPS.
Application
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Biochem/physiol Actions
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Preparation Note
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Other Notes
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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This Item | |||
|---|---|---|---|
| description purified by trichloroacetic acid extraction | description purified by trichloroacetic acid extraction | description ≥10,000 EU/mg, purified by phenol extraction | description purified by phenol extraction |
| biological source Escherichia coli (O26:B6) | biological source Escherichia coli (O55:B5) | biological source Escherichia coli (O26:B6) | biological source Escherichia coli (O127:B8) |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility water: soluble |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| color white to yellow | color white to yellow | color white to faint yellow | color white to faint yellow |
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Oral
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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