This solution is supplied as a solution of lysozyme at 10 mg/mL, in 25 mM sodium acetate, pH 4.5, with 50% glycerol.
L3790
Lysozyme from chicken egg white
10 mg/mL
Synonym(s):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
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| Size/SKU | Availability | Price |
|---|---|---|
10 x 1 mL | Check Cart for Availability | $154.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.56
EC Number:
MDL number:
Biological source:
chicken egg white
Concentration:
≥400000 units/mL, 10 mg/mL
$154.00
Check Cart for Availability
biological source
chicken egg white
Quality Segment
form
liquid
mol wt
single-chain 14.3 kDa
concentration
≥400000 units/mL, 10 mg/mL
technique(s)
cell based assay: suitable
input
bacteria and yeast sample(s)
suitability
suitable for cell lysis
UniProt accession no.
application(s)
cell analysis
shipped in
wet ice
storage temp.
−20°C
Gene Information
chicken ... LYZ(396218)
General description
Lysosyme is an enzyme found in egg white that breaks down the cell walls in bacteria enhancing the efficiency of DNA and protein extraction.
Application
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Suitable for the purification of both DNA and protein from bacteria.
Tested for suitability with CelLytic™ B in protein extraction from bacteria.
Biochem/physiol Actions
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.
Physical form
Ready to use solution:10 mg/mL lysozyme in 25 mM sodium acetate pH 4.5 with 50% glycerol.
Solution in 50% glycerol, 25 mM Sodium Acetate
Other Notes
One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
1 of 1
This Item | |||
|---|---|---|---|
| description 10 mg/mL | description For use as a marker in SDS-PAGE | description free of DNA contaminants, suitable for Microbiome research, lyophilized powder, protein ≥90%, ≥39,000 units/mg protein | description Lysobac™, recombinant, expressed in rice, ≥100,000 units/mg protein, lyophilized powder |
| technique(s) cell based assay: suitable | technique(s) - | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information - | Gene Information human ... LYZ(4069) |
| biological source chicken egg white | biological source chicken egg white | biological source chicken egg white | biological source human |
| concentration ≥400000 units/mL, 10 mg/mL | concentration - | concentration - | concentration - |
| form liquid | form powder | form powder | form lyophilized powder |
| suitability suitable for cell lysis | suitability - | suitability suitable for cell lysis | suitability suitable for cell lysis |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
WGK
WGK 2
PPE (personal protective equipment)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Hiya, Just want to ask what is the solution the lysozyme is dissolved in for this product?
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