This product is not tested for purity. Only the enzymatic activity in U/mL is provided. The certificate of analysis contains the release specifications.
L3916
L-Lactic Dehydrogenase from bovine heart
1000 units/mL
Synonym(s):
Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-617-8
MDL number:
EC Number:
Specific activity:
1000 units/mL
Pricing and availability is not currently available.
General description
LDH (lactic dehydrogenase) is a glycolytic enzyme. It is found in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It comprises of five isoenzyme forms. LDH possess a tetrameric structure.
Application
For use in enzymatic determination of lactate or pyruvate.
L-Lactic Dehydrogenase from bovine heart has been used:
- as a reference standard to measure the LDH (lactic dehydrogenase)
- to quench protein-bound NADH in cells
- to measure the lactate released by the cells in suspension
Biochem/physiol Actions
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
LDH (lactic dehydrogenase) helps in the reversible conversion of lactate to pyruvate.
Physical form
Suspension in 2.2 M ammonium sulfate
Other Notes
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
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This Item | |||
|---|---|---|---|
| description 1000 units/mL | description ≥90 U/mg | description lyophilized powder, ≥80 units/mg solid | description ≥600 units/mg protein (biuret), ammonium sulfate suspension |
| specific activity 1000 units/mL | specific activity ≥90 U/mg | specific activity ≥80 units/mg solid | specific activity ≥600 units/mg protein (biuret) |
| form solution | form powder | form lyophilized powder | form ammonium sulfate suspension |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 1
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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What is the protein mass (mg of protein/mL)? Also, could you provide data on the protein purity?
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