Key Documents

View All Documentation

Lysozyme from chicken egg white

Name: Lysozyme from chicken egg white
Brand: SIGMA
Price: 61 USD
Availability: InStock

BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein

Synonym(s):

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

Select a Size

Change View

Size/SKU	Availability	Price
500 mg	Available to ship TODAYfromMILWAUKEE	$61.00
1 g	Available to ship TODAYfromMILWAUKEE	$157.00
5 g	Available to ship TODAYfromMILWAUKEE	$524.00

About This Item

CAS Number:

12650-88-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

235-747-3

MDL number:

MFCD00131557

EC Number:

3.2.1.17 (BRENDA, IUBMB)

Specific activity:

≥40,000 units/mg protein

Assay:

≥98% (SDS-PAGE)

Biological source:

chicken egg white

$61.00

Available to ship TODAYDetails

Request a Custom Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

chicken egg white

Quality Segment

200

grade

Molecular Biology

product line

BioUltra

assay

≥98% (SDS-PAGE)

form

lyophilized powder

specific activity

≥40,000 units/mg protein

mol wt

single-chain 14.3 kDa

composition

Protein, ≥90%

technique(s)

cell based assay: suitable

input

bacteria and yeast sample(s)

suitability

suitable for cell lysis

application(s)

cell analysis

storage temp.

−20°C

Description

General description

Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.

Application

Lysozyme from chicken egg white has been used:

in dielectric spectroscopy studies of dynamics of protein
as a control to measure the human lysozyme activity
as a supplement in soaking solution to treat lenses

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Biochem/physiol Actions

Lysozymes participate in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Features and Benefits

Highly purified by repeated crystallization and dialysis
Each lot is use-tested for isolation of plasmid DNA from E. coli

Preparation Note

3× crystallized

Other Notes

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	L4631	L6876	L3790
Sigma-Aldrich L4919 Lysozyme from chicken egg white	Sigma-Aldrich L4631 Lysozyme from chicken egg white	Sigma-Aldrich L6876 Lysozyme from chicken egg white	Sigma-Aldrich L3790 Lysozyme from chicken egg white
description BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein	description For use as a marker in SDS-PAGE	description powder or granules, ≥39,000 units/mg protein	description 10 mg/mL
assay ≥98% (SDS-PAGE)	assay -	assay -	assay -
biological source chicken egg white	biological source chicken egg white	biological source chicken egg white	biological source chicken egg white
grade Molecular Biology	grade -	grade -	grade -
technique(s) cell based assay: suitable	technique(s) -	technique(s) cell based assay: suitable	technique(s) cell based assay: suitable
specific activity ≥40,000 units/mg protein	specific activity -	specific activity ≥39,000 units/mg protein	specific activity -
application(s) cell analysis	application(s) -	application(s) cell analysis	application(s) cell analysis

Still not finding the right product?

Explore all of our products under Lysozyme from chicken egg white

Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.