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Lipopolysaccharides (rough strains) from Escherichia coli J5 (Rc mutant)

Name: Lipopolysaccharides (rough strains) from <I>Escherichia coli</I> J5 (Rc mutant)
Brand: SIGMA

Synonym(s):

LPS

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About This Item

CAS Number:

93572-42-0

UNSPSC Code:

12352201

EC Number:

297-473-0

NACRES:

NA.25

MDL number:

MFCD00164401

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Properties

biological source

Escherichia coli (J5 Rc mutant)

Quality Segment

200

form

lyophilized powder

impurities

<3% Protein (Lowry)

color

white to faint beige

solubility

water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

Description

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

Prepared by phenol-chloroform-petroleum ether extraction.

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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This Item	L3023	L3755	L6893
Sigma-Aldrich L5014 Lipopolysaccharides (rough strains) from Escherichia coli J5 (Rc mutant)	Sigma-Aldrich L3023 Lipopolysaccharides from Escherichia coli O111:B4	Sigma-Aldrich L3755 Lipopolysaccharides from Escherichia coli O26:B6	Sigma-Aldrich L6893 Lipopolysaccharides (rough strains) from Escherichia coli F583 (Rd mutant)
description -	description Detoxified	description purified by trichloroacetic acid extraction	description -
biological source Escherichia coli (J5 Rc mutant)	biological source Escherichia coli (O111:B4)	biological source Escherichia coli (O26:B6)	biological source Escherichia coli (F583 Rd mutant)
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
solubility water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow	solubility water: soluble	solubility water: soluble	solubility water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
impurities <3% Protein (Lowry)	impurities -	impurities 1-10% Protein (Lowry)	impurities <3% Protein (Lowry)

pictograms

GHS06

signalword

Danger

hcodes

H300

pcodes

P264 - P270 - P301 + P310 - P405 - P501

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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