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Lipopolysaccharides from Escherichia coli O127:B8

Name: Lipopolysaccharides from <I>Escherichia coli</I> O127:B8
Brand: SIGMA
Price: 730 USD
Availability: InStock

purified by ion-exchange chromatography, TLR ligand tested

Synonym(s):

LPS

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Size/SKU	Availability	Price
10 mg	Available to ship TODAYfromMILWAUKEE	$730.00
25 mg	Available to ship TODAYfromMILWAUKEE	$1,410.00

About This Item

CAS Number:

93572-42-0

UNSPSC Code:

12352201

EC Number:

297-473-0

NACRES:

NA.25

MDL number:

MFCD00212998

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Properties

biological source

Escherichia coli (O127:B8)

Quality Segment

200

form

lyophilized powder

purified by

ion-exchange chromatography

impurities

<1% Protein, <1% RNA

color

white to yellow cast

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

Description

General description

This product is extracted from E. coli serotype O127:B8 and purified by ion exchange. The source strain is ATCC 12740. This LPS serotype has been used in the study of septic shock and to induce NOS in murine macrophages and PAF synthesis in rat glomerular masangial cells.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/mL) or cell culture medium (1 mg/mL) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/mL) has been achieved in aqueous saline after vortexing and warming to 70-80 °C. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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This Item	L4516	L3024	L2637
Sigma-Aldrich L5024 Lipopolysaccharides from Escherichia coli O127:B8	Sigma-Aldrich L4516 Lipopolysaccharides from Escherichia coli O127:B8	Sigma-Aldrich L3024 Lipopolysaccharides from Escherichia coli O111:B4	Sigma-Aldrich L2637 Lipopolysaccharides from Escherichia coli O55:B5
description purified by ion-exchange chromatography, TLR ligand tested	description BioXtra, suitable for cell culture, γ-irradiated	description purified by ion-exchange chromatography, TLR ligand tested	description purified by gel-filtration chromatography
biological source Escherichia coli (O127:B8)	biological source Escherichia coli (O127:B8)	biological source Escherichia coli (O111:B4)	biological source Escherichia coli (O55:B5)
solubility water: soluble	solubility H₂O: 5 mg/mL, slightly hazy	solubility water: soluble	solubility water: soluble
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
impurities <1% Protein, <1% RNA	impurities <1% Protein (Lowry)	impurities <1% RNA, <1% Protein	impurities <3% Protein

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Pictograms

GHS06

Signal Word

Danger

Hazard Codes

H300

Precautionary Statements

P264 - P270 - P301 + P310 - P405 - P501

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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