This product is a beta-lactamase blend containing different enzymes to cover a broad spectrum of beta-lactam antibiotic substrates. No specific molecular weight range is provided. However, information in BRENDA indicates that most of these recombinant enzymes fall within the range of approximately 30 kDa.
L6170
β-Lactamase
recombinant, expressed in E. coli
Synonym(s):
Carbapenemase, Cephalosprinase
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| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Check Cart for Availability | $783.00 |
$783.00
Check Cart for Availability
recombinant
expressed in E. coli
Quality Segment
form
powder
specific activity
≥20 U/mg (with cephalosporin C), ≥400 U/mg (with benzylpenicilin)
storage temp.
2-8°C
General description
β-Lactamase produced by bacteria is closely related to the penicillin binding proteins. β-Lactamase hydrolyzes β-lactum antibiotics and is the prime cause of resistance development by bacteria. There are four subclasses of β-Lactamases. Classes A, C and D form an acyl-enzyme via active site serine residue. Class B β-lactamases are metalloenzymes with zinc ion at their active site for β-lactam hydrolysis. Mutations in the β-lactamases has resulted in the generation of extended-spectrum β-lactamases (ESBLs). As close to 900 types of β-Lactamases are produced by microbes.
Application
β-Lactamase has been used in coumarin-cephalosporin-fluorescein-acetoxymethyl (CCF4)- β-Lactamase assay in dendritic cells and in the hydrolysis of meropenem (H-Mer) to generate 18O-labeled H-Mer.
β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product L6170 is recombinantly produced based on the sequence of the enzyme from Pseudomonas aeruginosa, and is expressed in E. coli.
Biochem/physiol Actions
β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring. Typical analysis for other substrates: ceftriaxon 2-4 un/mg; cefazolin 2-4 un/mg; ceftazidime 1.5-3 un/mg; cefoxitin 0.35-0.7 un/mg; cefepime 1.2-2.4 un/mg; cefuroxime 1.5-3 un/mg; cefotaxime 1-2 un/mg, oxacillin 4-8 un/mg; imipenem 1.2-2.4 un/mg; and meropenem 3-6 un/mg
Typical analysis for other substrates: ceftriaxon 2-4 un/mg; cefazolin 2-4 un/mg; ceftazidime 1.5-3 un/mg; cefoxitin 0.35-0.7 un/mg; cefepime 1.2-2.4 un/mg; cefuroxime 1.5-3 un/mg; cefotaxime 1-2 un/mg, oxacillin 4-8 un/mg; imipenem 1.2-2.4 un/mg; and meropenem 3-6 un/mg
Physical form
Lyophilized powder containing sodium chloride and potassium phosphate.
Other Notes
One unit will hydrolyze 1.0 μmole substrate per min (βI: benzylpenicillin; βII: cephalosporin C) in 50 mM phosphate buffer, pH 7.0 containing 10 μM zinc chloride at 25 ºC.
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This Item | |||
|---|---|---|---|
| description recombinant, expressed in E. coli | description recombinant, expressed in E. coli | description CENTA β-Lactamase Substrate is a chromogenic β-lactamase substrate for both Gram-negative and Gram-positive bacteria. | description ≤30% α-anomer basis, ≥99% total lactose basis |
| specific activity ≥20 U/mg (with cephalosporin C), ≥400 U/mg (with benzylpenicilin) | specific activity - | specific activity - | specific activity - |
| form powder | form lyophilized powder | form powder | form powder |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - | recombinant - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. room temp |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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What is the molecular weight and class of this beta-lactamase?
1 answer-
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Hi. I have a question about product L6170. Is the product in lyophilized form and if yes, how many grams of powder each vial contains?
1 answer-
Yes, this is a lyophilized product. However, the preparation includes sodium chloride and potassium phosphate salts. The amount of material is each vial varies from lot to lot and may be calculated based on analytical data reported in the Certificate of Analysis. For example, a lot that reports 1289 units per vial, 806 units per mg protein, and 0.87% protein, will contain 184 milligrams per vial based on the following calculation:
Units per vial / (units per mg protein x % protein) = milligrams per vial
Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/l6170#product-documentationHelpful?
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What is the volume needed to resuspend the lyophilized enzyme?
1 answer-
To reconstitute, it is recommended to dissolve the contents of one vial in 10 mL of deionized, purified water. After reconstitution, filter-sterilize through a 0.2 µm low-protein binding membrane.
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