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L7647

Concanavalin A from Canavalia ensiformis (Jack bean)

Type VI, lyophilized powder

Synonym(s):

Con A

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Size/SKUAvailabilityPrice
25 mg
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$35.90
100 mg
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$104.00
250 mg
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$175.00
1 g
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$564.00

About This Item

CAS Number:
UNSPSC Code:
12352202
EC Number:
234-258-2
NACRES:
NA.32
MDL number:
Form:
lyophilized powder
Biological source:
Canavalia ensiformis

$35.90


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biological source

Canavalia ensiformis

Quality Segment

type

Type VI

form

lyophilized powder

potency

≤20 μg per mL agglutination activity

impurities

Carbohydrate, essentially free, salt, essentially free

solubility

water: 1 % (w/v)

storage temp.

−20°C

Application

Concanavalin A from Canavalia ensiformis (Jack bean) has been used for developing zwitterion-immobilized molecularly imprinted polymers (ziMIPs) for protein crystallization. It has been also used in the preparation of protein loaded microgels to prevent enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Preparation Note

Highly purified by specific affinity chromatography.

Analysis Note

Agglutination activity: Determined from serial dilutions of a 1 mg/mL solution (this activity is the lowest conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Type A Human Blood.

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description

Type VI, lyophilized powder

description

Type IV, lyophilized powder

description

peroxidase conjugate, lyophilized powder

description

Type IV-S, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

biological source

Canavalia ensiformis

biological source

-

biological source

Canavalia ensiformis (jack bean)

biological source

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

impurities

Carbohydrate, essentially free, salt, essentially free

impurities

Carbohydrate, essentially free, salt, essentially free

impurities

carbohydrate, essentially free

impurities

salt, essentially free

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

water: 1 % (w/v)

solubility

-

solubility

-

solubility

PBS: 5 mg/mL, slightly hazy, H2O: soluble, slightly hazy

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200


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Pictograms

Health hazard

Signal Word

Danger

Hazard Codes

Hazard Classifications

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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Questions

  1. What is the molecular weight of Concanavalin A with code L7647?

    1 answer
    1. The molecular weight of Concanavalin A is 102 kDa. Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/169/422/l7647pis.pdf

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