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L7773

Lysozyme from chicken egg white

aseptically filled

Synonym(s):

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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Size/SKUAvailabilityPrice
50 mg

Available to ship TODAYfromMILWAUKEE

$354.00
$300.90

About This Item

CAS Number:
12650-88-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
235-747-3
MDL number:
MFCD00131557
EC Number:
3.2.1.17 (BRENDA, IUBMB)

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sterility

aseptically filled

Quality Segment

300

form

powder

enzyme activity

≥40000 units/mg protein

mol wt

single-chain 14.3 kDa

input

bacteria and yeast sample(s)

UniProt accession no.

P00698

storage temp.

−20°C

Gene Information

chicken ... LYZ(396218)

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.

Preparation Note

Prepared from L6876

Other Notes

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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This Item
L4631L6876L2879
Lysozyme from chicken egg white aseptically filled

Sigma-Aldrich

L7773

Lysozyme from chicken egg white

Lysozyme from chicken egg white For use as a marker in SDS-PAGE

Sigma-Aldrich

L4631

Lysozyme from chicken egg white

Lysozyme from chicken egg white powder or granules, ≥39,000 units/mg protein

Sigma-Aldrich

L6876

Lysozyme from chicken egg white

Lysozyme chloride form from chicken egg white Grade VI, ≥35,000 units/mg protein (E1%/282)

Sigma-Aldrich

L2879

Lysozyme chloride form from chicken egg white

description

aseptically filled

description

For use as a marker in SDS-PAGE

description

powder or granules, ≥39,000 units/mg protein

description

Grade VI, ≥35,000 units/mg protein (E1%/282)

Gene Information

chicken ... LYZ(396218)

Gene Information

chicken ... LYZ(396218)

Gene Information

chicken ... LYZ(396218)

Gene Information

-

form

powder

form

powder

form

powder or granules

form

lyophilized powder

sterility

aseptically filled

sterility

-

sterility

-

sterility

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

−20°C

UniProt accession no.

P00698

UniProt accession no.

P00698

UniProt accession no.

P00698

UniProt accession no.

-

mol wt

single-chain 14.3 kDa

mol wt

single-chain 14.3 kDa

mol wt

single-chain 14.3 kDa

mol wt

~14.3 kDa

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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