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L8258

Lectin from Wisteria floribunda

lyophilized powder

Synonym(s):

Wisteria floribunda agglutinin, WFA

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Size/SKUAvailabilityPrice
1 mg
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$109.00
5 mg

Available to ship TODAYfromMILWAUKEE

$347.00
$294.95

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
Form:
lyophilized powder

$109.00


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form

lyophilized powder

Quality Segment

potency

<16 μg/mL agglutination activity

composition

Protein, ~95% E1%/280

storage temp.

−20°C

General description

Lectin is a carbohydrate-binding protein found in plant roots, rhizomes, leaves, seeds, and stems. Lectin is located in the cytoplasm as well as in the nucleus.

Application

Lectin from Wisteria floribunda has been used:
  • to study the perineuronal nets and parvalbumin nets in mouse brain by immunofluorescence
  • to evaluate the amount of N-acetylgalactosamine (GalNAc) transferred to Gaussia luciferase (GLuc) substrates by microplate assay
  • to study its binding effects on perineuronal nets

Biochem/physiol Actions

Lectin binds with high affinity to the glycans of polysaccharides, glycolipids, and glycoproteins. It is involved in plant defense.
WFA is not blood group specific, but has an affinity for N-acetyl-D-galactosamine.

Packaging

Package size based on protein content

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

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L1516L1882L2895
description

lyophilized powder

description

buffered aqueous solution, biotin conjugate

description

agarose conjugate, saline suspension

description

Isolectin B4 (BSI-B4), FITC conjugate, lyophilized powder

form

lyophilized powder

form

buffered aqueous solution

form

saline suspension

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

potency

<16 μg/mL agglutination activity

potency

-

potency

-

potency

<50 μg per mL agglutination activity (using human blood group B erythrocytes)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Protein, ~95% E1%/280

composition

-

composition

-

composition

Protein, ≥70% Lowry (, balance sodium citrate)


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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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