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L8754

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder

Synonym(s):

Phaseolus vulgaris agglutinin, PHA

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Size/SKUAvailabilityPrice
5 mg

Available to ship TODAYfromMILWAUKEE

$38.40
25 mg

Available to ship TODAYfromMILWAUKEE

$107.00
50 mg

Available to ship TODAYfromMILWAUKEE

$188.00

About This Item

UNSPSC Code:
12352202
EC Number:
232-718-7
NACRES:
NA.32
Form:
lyophilized powder
Biological source:
Phaseolus vulgaris

$38.40

Available to ship TODAYDetails

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biological source

Phaseolus vulgaris

Quality Segment

form

lyophilized powder

potency

≤62.5 μg per mL agglutination activity, ≤10 μg/mL mitogenic activity

impurities

salt, free

storage temp.

2-8°C

General description

PHAs are carbohydrate-binding proteins. There are several types of PHAs identified, predominantly in leguminous plants.
Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants (esp. in legumes). It is obtained from Phaseolus vulgaris (red kidney beans) and acts as a mitogen in human leucocyte culture.

Application

It has also been used as an antigen to measure in vivo T-cell-mediated immune responsiveness.
Lectin from Phaseolus vulgaris (red kidney bean) has been used in phytohemagglutinin (PHA) assay and during assessment of cellular and humoral immunity.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Analysis Note

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.

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This Item
L2769L890261764
Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-P, lyophilized powder

Sigma-Aldrich

L8754

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Leucoagglutinin PHA-L, lyophilized powder

Sigma-Aldrich

L2769

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-M, lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

L8902

Lectin from Phaseolus vulgaris (red kidney bean)

Sigma-Aldrich

Sigma-Aldrich

61764

Lectin from Phaseolus vulgaris

description

Phytohemagglutinin PHA-P, lyophilized powder

description

Leucoagglutinin PHA-L, lyophilized powder

description

Phytohemagglutinin PHA-M, lyophilized powder, BioReagent, suitable for cell culture

description

lyophilized, powder

biological source

Phaseolus vulgaris

biological source

Phaseolus vulgaris

biological source

-

biological source

Phaseolus vulgaris

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

impurities

salt, free

impurities

salt, free

impurities

salt, free

impurities

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

potency

≤62.5 μg per mL agglutination activity, ≤10 μg/mL mitogenic activity

potency

≤5 μg/mL mitogenic activity, ≤1.0 μg/mL leucoagglutination activity, erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.)

potency

<40 μg per mL agglutination activity

potency

-

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

100

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Questions

1–2 of 2 Questions  

  1. Which if these Phytohaemagglutinin is most suitable for cell mediated immune response in chicken (wing web application). I'm interested in PHA-P, will it give me good results?

    1 answer

    1. This product, and other phytohemagglutinins, have not been tested for in vivo use. However, there are several publications citing this product, as well as product L9017, for use in the chicken wing web assay.

      Please see the link below to review additional information available in the product datasheet, including the lectin selection tables on pages 2 & 3:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/423/018/l8754pis.pdf
      Please see the following links to review example publications:
      https://www.sciencedirect.com/science/article/pii/S0032579119420300
      https://www.sciencedirect.com/science/article/pii/S0032579119311174
      https://www.nature.com/articles/s41598-017-08733-0
      https://besjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2435.2006.01094.x

      Helpful?

  2. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 answer

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

      Helpful?

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