This product is supplied by the ATCC. The growth phase at the time of harvest is not reported by the supplier. In general, in order to maximize production and viability, cells are in log phase or beginning stationary phase at the time of harvest.
M3770
Micrococcus lysodeikticus ATCC No. 4698
suitable for substrate for the assay of lysozyme, lyophilized cells
Synonym(s):
Micrococcus luetus
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| Size/SKU | Availability | Price |
|---|---|---|
5 g | Check Cart for Availability | $112.00 |
25 g | Check Cart for Availability | $469.00 |
100 g | Check Cart for Availability | $1,250.00 |
About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
$112.00
Check Cart for Availability
form
lyophilized cells
Quality Segment
suitability
suitable for substrate for the assay of lysozyme
storage temp.
−20°C
Application
Micrococcus lysodeikticus ATCC No. 4698 has been used in a study to assess lysozyme separation by hollow-fibre ultrafiltration. PMID 10908864 It has also been used in a study to investigate the encapsulation of protein drugs in biodegradable microparticles. PMID 17945246
Lysozyme lysates harvested from cultures of Micrococcus lysodeikticus were attached to sepharose and used for affinity chromatography to isolate various bacteriolytic enzymes. [1]
Biochem/physiol Actions
Micrococcus luetus is a Gram-positive bacteria that is identified by the release of yellow water-insoluble pigments. This species requires succinic acid for its growth and is found to be susceptible to β-lytic metalloendopeptidase lyses by Lysobacter enzymogenes. Its membrane includes enzymes that participate in the prenylation reactions by utilizing prenyl pyrophosphates as donors. M. luteus is known to be used for cloning the cis-prenyl transferase gene.
Analysis Note
Contains polynucleotide phosphorylase.
Other Notes
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
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This Item | |||
|---|---|---|---|
| description suitable for substrate for the assay of lysozyme, lyophilized cells | description lyophilized cells | description Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein | description 100-300 units/mg protein |
| suitability suitable for substrate for the assay of lysozyme | suitability - | suitability - | suitability - |
| form lyophilized cells | form lyophilized cells | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 400 | Quality Level 300 |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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From which growth phase are the bacteria harvested?
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