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M3817

MegaCell™ RPMI-1640 Medium

without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Size/SKUAvailabilityPrice
500 mL
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$76.40

About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

$76.40


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Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

General description

MegaCell™ is a line of versatile media, formulated to significantly reduce the amount of serum required for cultivating mammalian cells in vitro. It is effective as a basal formulation for the growth and maintenance of adherent or suspension cell lines. When supplemented with 3% fetal bovine serum (FBS), MegaCell supports proliferative rates and maximal cell densities comparable, and in some cases superior, to the conventional basal formulation supplemented with 10% fetal bovine serum.

Application

MegaCell™ RPMI-1640 Medium has been used:
  • as a component of the TSC medium for culturing trophoblast stem cells (TSC)
  • as a component of 25 ng/ml FGF4 and 1 g/ml heparin (TSF4H medium) for culturing TSCs
  • as a component of embryo-like structures (ETS) embryo medium and TS cell medium for culturing embryonic stem cells (ESCs) and TSCs

Preparation Note

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

Legal Information

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.

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R1780R6504R7513
description

without L-glutamine, liquid, sterile-filtered, suitable for cell culture

description

With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling for cell culture applications

description

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

description

Modified, with sodium bicarbonate, without methionine, cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

form

liquid

form

liquid

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no

components

NaHCO3: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L

components

L-glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no
phenol red: yes

components

HEPES: no
sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient


Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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