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M8822

MEK1, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

MAP2K1, MAPKK1, MKK1, PRKMK1

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Size/SKUAvailabilityPrice
10 μg
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$598.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
276-374 nmol/min·mg
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells

$598.00

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recombinant

expressed in baculovirus infected Sf9 cells

Quality Segment

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

276-374 nmol/min·mg

mol wt

~69 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... Map2k1(26395)

General description

Mitogen activated protein kinase/ERK kinase (MEK)1 is a hydrophilic non-receptor protein and is a member of dual specificity protein kinase family. It is ubiquitously expressed. MAP2K1 is encoded by the gene mapped to human chromosome 15q22.31.

Application

MEK1, active, GST tagged human has been used to induce kinase reactions in in vitro mitogen activated protein kinase/ERK kinase (MEK)1 kinase assay. It has also been used to activate extracellular-signal-regulated kinase 2 (ERK2) by phosphorylation.

Biochem/physiol Actions

Mitogen activated protein kinase/ERK kinase (MEK)1/2 catalyzes the phosphorylation of tyrosine and then threonine in extracellular-signal-regulated kinase1/2. As an essential component of RAS−RAF−MEK−ERK signaling pathway (MAP kinase signaling cascade), it is involved in the regulation of apoptosis, cell cycle progression, cell migration, differentiation, metabolism, and proliferation. Constitutive activation of MEK1 results in cellular transformation. Thus, MEK1 represents a likely target for pharmacologic intervention in proliferative diseases such as cancer.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

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MEK1, active, GST tagged human PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sigma-Aldrich

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MEK1, active, GST tagged human

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MST1, active, GST tagged human PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sigma-Aldrich

M9697

MST1, active, GST tagged human

Anti-MAP2K1 antibody produced in rabbit

Sigma-Aldrich

SAB5700083

Anti-MAP2K1 antibody produced in rabbit

description

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

description

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

description

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

description

-

specific activity

276-374 nmol/min·mg

specific activity

190-258 nmol/min·mg

specific activity

952-1288 nmol/min·mg

specific activity

-

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

-

Gene Information

human ... Map2k1(26395)

Gene Information

human ... MAP2K2(5605)

Gene Information

human ... STK4(6789)

Gene Information

human ... MAP2K1(5604)

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

-

mol wt

~69 kDa

mol wt

~71 kDa

mol wt

~83 kDa

mol wt

43, 40

Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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