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MAK169

High Sensitivity Pyrophosphate Assay Kit

sufficient for 200 fluorometric tests

Synonym(s):

Pyrophosphate (PPi) Assay Kit

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1 kit
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$746.00

About This Item

UNSPSC Code:
12161503
EC Number:
200-664-3
NACRES:
NA.84

$746.00

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usage

sufficient for 200 fluorometric tests

application(s)

cosmetics
food and beverages

detection method

fluorometric

relevant disease(s)

genitourinary disorders; rheumatological diseases

storage temp.

−20°C

General description

Pyrophosphate (PPi) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters.

Application

The Pyrophosphate Assay Kit provides a simple and direct flourescent microplate procedure for measuring pyrophosphate in a variety of samples.

Biochem/physiol Actions

The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λex =370/λem = 470 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.

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MAK168P7275
High Sensitivity Pyrophosphate Assay Kit sufficient for 200 fluorometric tests

Sigma-Aldrich

MAK169

High Sensitivity Pyrophosphate Assay Kit

Pyrophosphate Assay Kit sufficient for 200 fluorometric tests (Blue fluorescence)

Sigma-Aldrich

MAK168

Pyrophosphate Assay Kit

Pyrophosphate reagent spectrophotometric assay

Sigma-Aldrich

P7275

Pyrophosphate reagent

description

sufficient for 200 fluorometric tests

description

sufficient for 200 fluorometric tests (Blue fluorescence)

description

spectrophotometric assay

detection method

fluorometric

detection method

fluorometric

detection method

-

usage

sufficient for 200 fluorometric tests

usage

sufficient for 200 fluorometric tests (Blue fluorescence)

usage

 vial sufficient for ≤4 assays

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

application(s)

cosmetics
food and beverages

application(s)

cosmetics
food and beverages

application(s)

-

relevant disease(s)

genitourinary disorders; rheumatological diseases

relevant disease(s)

-

relevant disease(s)

-

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Codes

H318

Precautionary Statements

P280 - P305 + P351 + P338

Hazard Classifications

Eye Dam. 1

Storage Class

10 - Combustible liquids

WGK

WGK 3

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Questions

1–2 of 2 Questions  

  1. Can this kit be used to quantify pyrophosphate in cell extracts? If so, are there any recommendations for sample preparation?

    1 answer

    1. The kit has not been validated for use with cell lysates. No supporting data are currently available for this sample type. The end user would have to determine suitability. Please refer to the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/414/749/mak169bul.pdf

      Please see the link below to review product C3228, CelLytic Cell Lysis Reagent for use with Mammalian cell/tissues:
      https://www.sigmaaldrich.com/US/en/product/sigma/c3228

      Please see the following link to review the C3228 cell lysis protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/317/238/c3228bul.pdf

      Helpful?

  2. The product info sheet recommends 10-30min incubation after probe addition. Is this kit therefore amenable to continuous read formats or is signal development slow? Also, is there a recommended quenching method for this kit?

    1 answer

    1. This kit functions using an endpoint determination. Fluorescence intensity should be measured a single time between 10 and 30 minutes after the addition of the master reaction mix. The reaction signal remains stable for 2 hours. No recommended quenching method is available.

      Helpful?

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