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MP0050

Lookout® One-Step Mycoplasma PCR Detection Kit

Detection of Mycoplasma Contamination by conventional PCR

Synonym(s):

Mycoplasma Testing Kit

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25 test

Available to ship TODAYfromMILWAUKEE

$312.00
100 test

Available to ship TODAYfromMILWAUKEE

$904.00

About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

$312.00

Available to ship TODAYDetails

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Quality Level

100

recombinant

expressed in E. coli Taq

form

liquid, lyophilized

parameter

2 μL sample volume

shipped in

ambient

storage temp.

2-8°C

General description

Lookout® One-Step Mycoplasma PCR Detection Kit is a kit for direct detection of mycoplasma contaminations in cell cultures, cell culture media, and other biological matrices by conventional PCR. Designed to require minimal pipetting, the kit includes all reagents required for PCR: primers, nucleotides, polymerase and the internal amplification control, which are provided in a ready-to-use lyophilized reaction mix. Upon rehydration with the included buffer, the mix is ready to be dispensed into the reaction tubes according to the sample number. Only samples or controls need to be added prior to PCR.

Application

Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

Biochem/physiol Actions

Type of PCR:
For conventional, endpoint PCR with gel electrophoresis
Result Evaluation: Gel electrophoresis at endpoint of PCR

Preparation Note

Lyophilized OneStep Mix is reconstituted in 600μl Rehydration Buffer; Lyophilized Positive Control DNA is reconstituted in 300μl PCR Grade Water
Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. Once rehydrated, the components must be stored at ≤ -18 °C.

Other Notes

Kit Components:
Lyophilized Mix (Primer sets / Nucleotides / Internal Control DNA and polymerase at optimized concentrations in aliquots of 25 or 100 reactions each Rehydration Buffer)
Lyophilized Positive Control DNA
PCR Grade Water
Required Consumables:
Agarose gels and DNA stains
PCR reaction tubes and pipette filter tips

Required Lab Devices:
PCR cycler for conventional PCR
Gel electrophoresis and documentation system
Pipetting equipment
Microcentrifuge

Legal Information

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lookout® One-Step Mycoplasma PCR Detection Kit Detection of Mycoplasma Contamination by conventional PCR

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form

liquid, lyophilized

form

-

form

-

form

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

parameter

2 μL sample volume

parameter

-

parameter

-

parameter

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

recombinant

expressed in E. coli Taq

recombinant

-

recombinant

-

recombinant

-

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

Storage Class

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCZ5343
MKCZ5532
MKCZ3639
MKCZ1735
MKCX9826

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Global Trade Item Number

SKUGTIN
MP0050-25TST04065268063187
MP0050-100TST04065268063170

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