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MP0050

Lookout® One-Step Mycoplasma PCR Detection Kit

Detection of Mycoplasma Contamination by conventional PCR

Synonym(s):

Mycoplasma Testing Kit

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25 TEST

$312.00

100 TEST

$904.00

$312.00

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

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Quality Level

100

recombinant

expressed in E. coli Taq

form

liquid, lyophilized

parameter

2 μL sample volume

shipped in

ambient

storage temp.

2-8°C

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Lookout® One-Step Mycoplasma PCR Detection Kit Detection of Mycoplasma Contamination by conventional PCR

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Sigma-Aldrich

MP0035

LookOut® Mycoplasma PCR Detection Kit

recombinant

expressed in E. coli Taq

recombinant

-

recombinant

-

recombinant

-

form

liquid

form

-

form

-

form

-

parameter

2 μL sample volume

parameter

-

parameter

-

parameter

-

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

General description

Lookout® One-Step Mycoplasma PCR Detection Kit is a kit for direct detection of mycoplasma contaminations in cell cultures, cell culture media, and other biological matrices by conventional PCR. Designed to require minimal pipetting, the kit includes all reagents required for PCR: primers, nucleotides, polymerase and the internal amplification control, which are provided in a ready-to-use lyophilized reaction mix. Upon rehydration with the included buffer, the mix is ready to be dispensed into the reaction tubes according to the sample number. Only samples or controls need to be added prior to PCR.

Application

Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

Biochem/physiol Actions

Type of PCR:
For conventional, endpoint PCR with gel electrophoresis
Result Evaluation: Gel electrophoresis at endpoint of PCR

Preparation Note

Lyophilized OneStep Mix is reconstituted in 600μl Rehydration Buffer; Lyophilized Positive Control DNA is reconstituted in 300μl PCR Grade Water
Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. Once rehydrated, the components must be stored at ≤ -18 °C.

Other Notes

Kit Components:
Lyophilized Mix (Primer sets / Nucleotides / Internal Control DNA and polymerase at optimized concentrations in aliquots of 25 or 100 reactions each Rehydration Buffer)
Lyophilized Positive Control DNA
PCR Grade Water
Required Consumables:
Agarose gels and DNA stains
PCR reaction tubes and pipette filter tips

Required Lab Devices:
PCR cycler for conventional PCR
Gel electrophoresis and documentation system
Pipetting equipment
Microcentrifuge

Legal Information

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCZ5343
MKCZ1735
MKCZ5532
MKCZ3639
MKCX9826

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Articles

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Mycoplasma Detection, Prevention, and Elimination in Cell Culture

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

Cell Culture Fundamentals: Safety Aspects of Cell Culture

Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Global Trade Item Number

SKUGTIN
MP0050-100TST04065268063170
MP0050-25TST04065268063187

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