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Anti-Nitrotyrosine antibody produced in rabbit

Name: Anti-Nitrotyrosine antibody produced in rabbit
Brand: SIGMA
Price: 192 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Nitrotyrosine Antibody, Nitrotyrosine Antibody - Anti-Nitrotyrosine antibody produced in rabbit

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Size/SKU	Availability	Price
25 μL	Available to ship TODAYfromMILWAUKEE	$192.00
0.2 mL	Estimated to ship onNovember 09, 2026fromMILWAUKEE	$731.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01322409

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, ELISA (i), IF, IHC (f), WB

Citations:

$192.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

wide range

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (frozen sections): 1:1000 using methacarn-fixed, frozen sections of human Alzheimer′s Disease brain, indirect ELISA: 1:1,000 using nitrated-BSA, indirect immunofluorescence: 1:1,000 using A549 cells metabolically labeled with 3-nitrotyrosine, microarray: suitable, western blot: 1:1,000 using nitrated-BSA

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NOS1(4842)

Description

General description

Peroxinitrite (ONOO-), a potent oxidant linked to cellular oxidative damage, is an important member of the family of reactive oxygen and nitrogen species, rapidly generated in vivo from nitric oxide and superoxide anion. Nitrotyrosine is a product of tyrosine nitration, that is facilitated by reactive nitrogen species.

Immunogen

nitrated KLH (Keyhole Limpet Hemocyanin).

Application

Anti-Nitrotyrosine antibody produced in rabbit has been used in:

immunoblotting
enzyme-linked immunosorbent assay (ELISA)
immunohistochemistry
immunofluorescence immunocytochemistry
confocal microscopy
immunofluorescent staining

Biochem/physiol Actions

Anti-Nitrotyrosine recognizes nitrated proteins and 3-nitro-L-tyrosine. It does not cross-react with L-tyrosine, ρ-nitro-L-phenylalanine, 3-amino-L-tyrosine, 3-chloro-L-tyrosine and phospho-L-tyrosine BSA conjugates.

Peroxynitrite has been shown to promote the nitration of tyrosine residues in proteins, and oxidation of redox metal centers, DNA, lipids and cysteine and methionine residues in proteins, suggesting that it plays a major role in oxidative cellular damage. Peroxynitrite has been implicated in the pathogenesis of several inflammatory, infectious and degenerative human disease, including neurological conditions such as Alzheimer′s disease, amyotrophic lateral sclerosis (ALS), in the pathogenesis of asthma, atherosclerosis and a variety of conditions precipitated by endothelial injury.

Reacts with peroxynitrite-nitrated proteins and 3-nitro-L-tyrosine. Does not crossreact with L-tyrosine, p-nitro-L-phenylalanine, 3-amino-L-tyrosine, 3-chloro-L-tyrosine, and phospho-L-tyrosine. Binding of the antibody is blocked by 3-nitrotyrosine.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Purified by affinity chromatography on a column of 3-nitro-L-tyrosine.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Sigma-Aldrich N0409 Anti-Nitrotyrosine antibody produced in rabbit	$Anti-Neurofilament 200 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich N4142 Anti-Neurofilament 200 antibody produced in rabbit	Sigma-Aldrich F3648 Anti-Fibronectin antibody produced in rabbit	Sigma-Aldrich N3908 Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form affinity isolated antibody
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
technique(s) immunohistochemistry (frozen sections): 1:1000 using methacarn-fixed, frozen sections of human Alzheimer′s Disease brain, indirect immunofluorescence: 1:1,000 using A549 cells metabolically labeled with 3-nitrotyrosine, indirect ELISA: 1:1,000 using nitrated-BSA, western blot: 1:1,000 using nitrated-BSA, microarray: suitable	technique(s) microarray: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using animal cerebellum sections, western blot: suitable	technique(s) western blot: 1:1,000 using human plasma fibronectin, indirect ELISA: 1:10,000 using human fibronectin, indirect immunofluorescence: 1:400 using human foreskin cultured fibroblasts, microarray: suitable	technique(s) microarray: suitable, indirect immunofluorescence: 1:500 using NGF-treated PC12 cells, western blot: 1:5,000 using whole cell extract of NGF-treated PC12 cells, immunohistochemistry (frozen sections): 1:10,000 using rat brain

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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