No, all the cocktails are EDTA-free. Alternative metalloprotease inhibitors are included in the cocktail when needed, depending on the sample type. EDTA can be added if it does not interfere with the downstream purification or activity of your protein of interest. Please explore the reagent offerings below for recommended, ready-to-use EDTA products.
P0001
Phosphatase Inhibitor Cocktail
ReadyShield® ready-to-use solution, for the inhibition of Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase
Synonym(s):
Phosphatase inhibitor cocktail
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Check Cart for Availability | $128.00 |
5 mL | Check Cart for Availability | $423.00 |
About This Item
UNSPSC Code:
12352200
NACRES:
NA.54
Form:
ready-to-use solution
Solubility:
water: soluble
Storage temp.:
−20°C
$128.00
Check Cart for Availability
Product Name
ReadyShield® Phosphatase Inhibitor Cocktail, For Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase
Quality Segment
form
ready-to-use solution
packaging
pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)
input
mammalian sample(s)
solubility
water: soluble
storage temp.
−20°C
General description
This phosphatase Inhibitor Cocktail contains inhibitors with a broad specificity for L-isozymes of alkaline phosphatases, and serine/ threonine protein phosphatases. Phosphatase Inhibitor Cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.
This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield® P0001 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P0044.
This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield® P0001 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P0044.
Features and Benefits
- Does not freeze in storage for quick, convenient use.
- Specific for Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase.
- Suggested as 100 X solution.
- Reclosable screw-top vials.
- Can be stored in cooler for approx. 1 month or room temperature for a week.
Preparation Note
This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| description For Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase | description Safe for Proteomic Mass Spectrometry applications | description non-freezing solution | description for use in purification of Histidine-tagged proteins, Non-freezing solution |
| form ready-to-use solution | form ready-to-use solution | form ready-to-use solution | form ready-to-use solution |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility water: soluble |
| packaging pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial) | packaging - | packaging pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial) | packaging pkg of 5 mL (in amber polypropylene vial), pkg of 1 mL (in amber polypropylene vial) |
| input mammalian sample(s) | input general use sample(s) | input mammalian sample(s) | input general use sample(s) |
Storage Class
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Do ReadyShield® inhibitor cocktails include EDTA?
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What is the recommended storage temperature for ReadyShield® products?
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The product is stable at -20 °C for 2 years from the assay date, which can be found on the lot specific CoA. The solution will not freeze in -20 °C storage and can be used directly from the freezer. Our experts have collected additional stability information that show ReadyShield® products are stable for at least one month at 4 °C, or for at least one week at 25 °C (room temperature).
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