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Monoclonal Anti-PSF antibody produced in mouse

Name: Monoclonal Anti-PSF antibody produced in mouse
Brand: SIGMA
Price: 189 USD

clone B92, purified from hybridoma cell culture

Synonym(s):

Mouse Anti-Polypyrimidine tract-binding protein-associated splicing factor

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Size/SKU	Availability	Price
25 μL	Check Cart for Availability	$189.00
100 μL	Check Cart for Availability	$697.00 $592.45
0.2 mL	Check Cart for Availability	$748.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03457573

Conjugate:

unconjugated

Clone:

B92, monoclonal

Application:

ARR, ICC, IHC (f), IP, RIA, WB

Citations:

$189.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B92, monoclonal

form

buffered aqueous solution

species reactivity

mouse, human

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, microarray: suitable, radioimmunoassay: suitable using cells, western blot: 2-4 μg/mL using whole extract of cultured HeLa cells

isotype

IgG1

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TAP1(6890)

Description

General description

Monoclonal Anti-PSF (mouse IgG1 isotype) is derived from the B92 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a C57BL/6J mouse immunized with a lysate of mouse bone marrow-derived stromal cell line. Polypyrimidine tract-binding protein-associated splicing factor (PSF) is an ubiquitous nuclear matrix protein. It is characterized with two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues.

Immunogen

lysate of mouse bone marrow-derived stromal cell line.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)

Monoclonal Anti-PSF antibody produced in mouse has been used in:

immunofluorescence
immunohistochemistry
immunoprecipitation

Biochem/physiol Actions

Polypyrimidine tract-binding protein-associated splicing factor (PSF) plays a pivotal role in mammalian spliceosome. PSF is also necessary for pre-mRNA splicing and transcription. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	F8264	I2136	H6030
Sigma-Aldrich P2860 Monoclonal Anti-PSF antibody produced in mouse	Sigma-Aldrich F8264 Anti-Mouse IgG (γ-chain specific)−FITC antibody produced in goat	Sigma-Aldrich I2136 Anti-Human IgG (Fc specific) antibody produced in goat	Sigma-Aldrich H6030 Anti-HSV antibody produced in rabbit
description clone B92, purified from hybridoma cell culture	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
biological source mouse	biological source goat	biological source goat	biological source rabbit
Gene Information human ... TAP1(6890)	Gene Information -	Gene Information -	Gene Information -
species reactivity mouse, human	species reactivity -	species reactivity -	species reactivity -
antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
clone B92, monoclonal	clone polyclonal	clone polyclonal	clone polyclonal
conjugate unconjugated	conjugate FITC conjugate	conjugate unconjugated	conjugate unconjugated

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Storage Class

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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