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Anti-Protein Kinase Cα antibody produced in rabbit

Name: Anti-Protein Kinase Cα antibody produced in rabbit
Brand: SIGMA
Price: 532 USD
Availability: InStock

whole antiserum

Synonym(s):

Anti-PKCα

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$532.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD00162769

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, WB

Citations:

135

$532.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen 80 kDa

contains

15 mM sodium azide

species reactivity

mouse, rat

technique(s)

microarray: suitable, western blot: 1:20,000 using mouse NIH 3T3 fibroblast lysate, western blot: 1:50,000 using rat brain extract

UniProt accession no.

P05696

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Prkca(18750)
rat ... Prkca(24680)

Description

General description

Anti-Protein Kinase Cα is produced in rabbit using a synthetic peptide conjugated to keyhole limpet hemocyanin (KLH) as the immunogen. PKC is a phospholipid dependent enzyme, activated by the lipid 1,2-diacylglycerol (DAG), an intracellular second messenger produced from hydrolysis of inositol phospholipids, in response to a variety of hormones, growth factors, and neurotransmitters.

PKC α is a conventional isoform of Protein Kinase C (76-93 kD) and a member of serine/threonine (Ser/Thr) protein kinases. It is expressed ubiquitously in tissues and is the major isoform of PKC in fibroblasts. Stimulation and overexpression of PKC α increases the growth rate of cells in culture. Anti-Protein Kinase C α antibody is useful for studying expression of PKC α in normal and neoplastic tissues. It is also useful for detecting PKC α isoenzyme in brain tissue and cell extracts. Furthermore, it can be used in chemiluminescence detection systems to detect PKC α. Rabbit anti-protein kinase c α antibody reacts specifically with PKC α (80 kD) of rat brain extract or NIH3T3 mouse fibroblast lysate.

Immunogen

Synthetic peptide corresponding to amino acids from the C-terminal variable (V5) region of rat PKC α conjugated to KLH.

Application

Anti-Protein Kinase C α has been used in immunoblotting and immunohistochemistry.

Anti-Protein Kinase Cα antibody produced in rabbit has been used in light microscopic—immunocytochemical analysis and immunofluorescent labelling.

Biochem/physiol Actions

Protein Kinase C (PKC) plays a crucial role in signal transduction leading to cellular regulation, cell growth and differentiation, oncogenesis and modulation of neurotransmission. PKC is a phospholipid dependent enzyme, activated by the lipid 1,2-diacylglycerol (DAG), an intracellular second messenger produced as a result from hydrolysis of inositol phospholipids, in response to a variety of hormones, growth factors and neurotransmitters. PKC is also the major cellular receptor for the tumor promoting phorbol esters. PKC action is mediated by binding to specific receptors for activated C-kinase (RACKs) and through the phosphorylation of several cellular substrates. Overexpression and stimulation of PKC α leads to enhanced growth rate of cells in culture. Anti-Protein Kinase C α antibody is useful for studying expression of PKC α in normal and neoplastic tissues.

The antibody reacts in immunoblotting with PKCα in rat brain extract. Staining is specifically inhibited with PKCα immunizing peptide (659-672).

Physical form

Supplied as a liquid containing 15 mM sodium azide as preservative

Preparation Note

For continuous use, store at 2-8 °C. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	P8333	P5704	M5670
Sigma-Aldrich P4334 Anti-Protein Kinase Cα antibody produced in rabbit	Sigma-Aldrich P8333 Anti-Protein Kinase Cδ antibody produced in rabbit	Sigma-Aldrich P5704 Monoclonal Anti-Protein Kinase C (PKC) antibody produced in mouse	Sigma-Aldrich M5670 Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit
description whole antiserum	description whole antiserum	description clone MC5, ascites fluid	description whole antiserum
antibody form whole antiserum	antibody form whole antiserum	antibody form ascites fluid	antibody form whole antiserum
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
clone polyclonal	clone polyclonal	clone MC5, monoclonal	clone polyclonal
technique(s) microarray: suitable, western blot: 1:50,000 using rat brain extract, western blot: 1:20,000 using mouse NIH 3T3 fibroblast lysate	technique(s) dot blot: 1:50,000, microarray: suitable, western blot: 1:10,000 using rat brain extract	technique(s) immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, microarray: suitable, western blot: 1:500 using rat brain cytosol preparation	technique(s) microarray: suitable, western blot: 1:40,000-1:20,000 using rat brain extract and mouse NIH 3T3 fibroblast extract, respectively

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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Anti-Protein Kinase Cα antibody produced in rabbit

whole antiserum

Select a Size

About This Item

P4334-.2ML

$532.00

biological source

Quality Segment

conjugate

antibody form

antibody product type

clone

mol wt

contains

species reactivity

technique(s)

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Gene Information

General description

Immunogen

Application

Biochem/physiol Actions

Physical form

Preparation Note

Disclaimer

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Safety Information

Storage Class

wgk

flash_point_f

flash_point_c

Documentation

Certificates of Analysis (COA)

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