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P4664

o-Phenylenediamine dihydrochloride

chromogenic, tablet

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Synonym(s):

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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50 tabs
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$284.00
100 tabs
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$488.00

About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
Molecular Weight:
181.06
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
3912045

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Product Name

o-Phenylenediamine dihydrochloride, tablet, 15 mg substrate per tablet

Quality Segment

form

tablet

technique(s)

protein detection: suitable

mp

258 °C (dec.) (lit.)

solubility

water: 1 tablet/10 mL, clear, colorless

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine dihydrochloride has been used:
  • as a substrate in conventional capture enzyme-linked immunosorbent assay (ELISA) for recombinant hemagglutinin (rHA) protein detection
  • as a substrate for the determination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulin G (IgG) antibodies by ELISA
  • as a colorimetric reagent specific for horseradish peroxidase (HRP) activity assessment in an ELISA protocol using SARS-CoV-2 antigens for S protein

Preparation Note

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

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This Item
P8806P7288P8412
description

tablet, 15 mg substrate per tablet

description

tablet, 3 mg substrate per tablet

description

tablet, 20 mg substrate per tablet

description

tablet, 30 mg substrate per tablet

solubility

water: 1 tablet/10 mL, clear, colorless

solubility

-

solubility

-

solubility

water: 1 tablet/10 mL, clear, colorless

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

tablet

form

tablet

form

tablet

form

tablet

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)


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Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges



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