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P5511

Protein Kinase A from bovine heart

≥0.8 units/μg protein, lyophilized powder

Synonym(s):

Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart

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1 mg

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$58.00
5 mg

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$157.00
10 mg

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$268.00

About This Item

CAS Number:
9026-43-1
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.77
EC Number:
2.7.1.37 (BRENDA, IUBMB)
MDL number:
MFCD00132121

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form

lyophilized powder

Quality Segment

specific activity

≥0.8 units/μg protein

composition

Protein, ≥70%

UniProt accession no.

storage temp.

−20°C

Gene Information

General description

Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme.

Application

Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.
Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin.

Biochem/physiol Actions

Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. It regulates apoptosis, mitochondrial respiration and ATP synthesis. PKA is modulated by protease calpain.
Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.

Packaging

Package size based on protein content

Physical form

Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.

Preparation Note

Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).

Analysis Note

Protein determined by biuret.

Other Notes

One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C in the presence of cyclic AMP, determined by measuring the production of ADP.

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This Item
P1431P2645C8482
Protein Kinase A from bovine heart ≥0.8 units/μg protein, lyophilized powder

Sigma-Aldrich

P5511

Protein Kinase A from bovine heart

Calmodulin from bovine testes BioUltra, ≥98% (SDS-PAGE), lyophilized powder, essentially salt free

Sigma-Aldrich

P1431

Calmodulin from bovine testes

Protein Kinase A Catalytic Subunit from bovine heart ≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)

Sigma-Aldrich

P2645

Protein Kinase A Catalytic Subunit from bovine heart

Protein Kinase A catalytic subunit human >90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Sigma-Aldrich

C8482

Protein Kinase A catalytic subunit human

description

≥0.8 units/μg protein, lyophilized powder

description

BioUltra, ≥98% (SDS-PAGE), lyophilized powder, essentially salt free

description

≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)

description

>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized (white powder to sticky mass to hard pellet)

form

buffered aqueous glycerol solution

UniProt accession no.

P05131, P00517

UniProt accession no.

P62157

UniProt accession no.

-

UniProt accession no.

P17612

Gene Information

cow ... PRKACA(282322), PRKACB(282323)

Gene Information

cow ... CALM3(520277)

Gene Information

-

Gene Information

human ... PRKACA(5566)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

specific activity

≥0.8 units/μg protein

specific activity

-

specific activity

≥9 units/μg protein (cyclic-AMP is not required for this activity)

specific activity

>1000 units/mg protein

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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