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P7623

PKD2 Active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), aqueous glycerol solution

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Synonym(s):

Protein Kinase D2

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5 μg
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$575.00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
MFCD07371065
Specific activity:
100 units/mg protein (Bradford)
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in E. coli

$575.00

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recombinant

expressed in E. coli

Quality Segment

200

assay

≥70% (SDS-PAGE)

form

aqueous glycerol solution

specific activity

100 units/mg protein (Bradford)

mol wt

protein apparent mol wt ~140 kDa

UniProt accession no.

Q9BZL6

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKD2(25865)

Application

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBTIDE substrate peptide (KRREILSRRPSYR), per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.

Biochem/physiol Actions

PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase that contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain. The PKD family of enzymes have been implicated in very diverse cellular functions, including Golgi organization and plasma membrane directed transport, metastasis, immune responses, apoptosis and cell proliferation.

Physical form

Solution of 50 μl in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.

Other Notes

One unit will incorporate one nanomole of phosphate into the CREBTIDE substrate (KRREILSRRPSYR) per minute at 30 °C at pH 7.2 using a final concentration of 50 μM [32P] ATP.

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PKD2 Active human recombinant, expressed in E. coli, ≥70% (SDS-PAGE), aqueous glycerol solution

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Sigma-Aldrich

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PKCμ, active, GST tagged human PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sigma-Aldrich

K4268

PKCμ, active, GST tagged human

description

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), aqueous glycerol solution

description

≥75% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

description

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

description

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

assay

≥70% (SDS-PAGE)

assay

≥75% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

Gene Information

human ... PRKD2(25865)

Gene Information

human ... PAK7(57144)

Gene Information

human ... PRKACB(5567)

Gene Information

human ... PRKD1(5587)

specific activity

100 units/mg protein (Bradford)

specific activity

10 units/mg protein (Bradford)

specific activity

≥10 nmol/min·mg

specific activity

578-782 nmol/min·mg

form

aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Storage Class

10 - Combustible liquids

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    Certificates of Analysis (COA)

    Lot/Batch Number
    045K0898

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