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P7643

PIPES dipotassium salt

≥99% (titration)

Synonym(s):

1,4-Piperazinediethanesulfonic acid dipotassium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) dipotassium salt

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Pack SizeSKUAvailabilityPrice
100 g
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$252.00

About This Item

Empirical Formula (Hill Notation):
C8H16K2N2O6S2
CAS Number:
108321-27-3
Molecular Weight:
378.55
NACRES:
NA.25
PubChem Substance ID:
24898738
UNSPSC Code:
12161700
MDL number:
MFCD00069751

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Quality Level

200

assay

≥99% (titration)

form

crystalline powder

concentration

0.1 M

useful pH range

6.1-7.5

pKa (25 °C)

6.8

solubility

water: 0.25 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

[K+].[K+].[O-]S(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O6S2.2K/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;;/h1-8H2,(H,11,12,13)(H,14,15,16);;/q;2*+1/p-2

InChI key

BJQYFCAMUXGZFN-UHFFFAOYSA-L

General description

PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.

Application

PIPES (1,4-Piperazinediethanesulfonic acid) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding. It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells. It is also suitable for use as buffer for purification of tubulin.

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assay

≥99% (titration)

assay

≥99% (titration)

assay

≥99% (titration)

assay

≥99% (titration)

Quality Level

200

Quality Level

400

Quality Level

200

Quality Level

400

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

form

crystalline powder

form

crystalline powder

form

crystalline powder

form

powder

solubility

water: 0.25 g/mL, clear, colorless

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless

solubility

water: 0.5 g/mL, clear, colorless

solubility

1 M NaOH: 0.5 M at 20 °C, clear, colorless

useful pH range

6.1-7.5

useful pH range

6.1-7.5

useful pH range

6.1-7.5

useful pH range

6.1-7.5

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000305931
0000305931
SLCH9283
0000268147
0000268147

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Helical alignment inversion of microtubules in accordance with a structural change in their lattice
Shikinaka K, et al.
Soft Matter, 11(19), 3869-3874 (2015)
Use of single molecule fluorescence polarization microscopy to study protein conformation and dynamics of kinesin-microtubule complexes
Benoit MPMH and Sosa H
Single Molecule Analysis, 15(1), 199-216 (2018)
Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation
Nie J, et al.
BMC Developmental Biology, 15(1), 10-10 (2015)
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Global Trade Item Number

SKUGTIN
P7643-1KG04061826186640
P7643-100G04061832549965
P7643-500G04061832549989
P7643-25G04061832549972

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