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P7828

Percoll®

suitable for plant cell culture

Synonym(s):

density gradient medium

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25 mL

Available to ship TODAYfromMILWAUKEE

$116.00
100 mL

Available to ship TODAYfromMILWAUKEE

$196.00
500 mL

Available to ship TODAYfromMILWAUKEE

$710.00

About This Item

UNSPSC Code:
10171502
NACRES:
NA.72
MDL number:
MFCD00131830

$116.00

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sterility

aseptically filled

Quality Level

200

form

colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP))

technique(s)

cell culture | plant: suitable

pH

8.9 (20 °C)

application(s)

agriculture

storage temp.

2-8°C

Application

Percoll® has been used in the density gradient centrifugation isolation of intact chloroplasts from green alga, decidual stromal cells and distinct populations of rooster sperm.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.
Percoll is used in plant research for protoplast and organelle (chloroplast, mitochondria) separation.

Legal Information

Percoll is a registered trademark of Cytiva

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Percoll® suitable for plant cell culture

Sigma-Aldrich

P7828

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Sigma-Aldrich

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Percoll® pH 8.5-9.5 (20 °C)

Sigma-Aldrich

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Phytamax™ Orchid Maintenance Medium powder, suitable for plant cell culture

Sigma-Aldrich

P6668

Phytamax™ Orchid Maintenance Medium

form

colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP))

form

colloidal

form

colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) )

form

powder

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | plant: suitable

sterility

aseptically filled

sterility

aseptically filled

sterility

aseptically filled

sterility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

application(s)

agriculture

application(s)

-

application(s)

hematology
histology

application(s)

agriculture

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000504178
0000504178
0000493084
0000493084
0000470036

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Protocols

How to Make and Use Percoll Gradients

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

Guangkun Yin et al.
Plant & cell physiology, 50(7), 1305-1318 (2009-06-13)
The development of mitochondria during seed germination is essential for plant growth. However, the developmental process is still poorly understood. Temperature plays a key role in soybean germination, and in this study we characterized the mitochondrial ultrastructure and proteome after
A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii
Mason CB, et al.
Nature Protocols, 1(5), 2227-2227 (2006)
Xiang-Jie Yin et al.
Clinical proteomics, 18(1), 9-9 (2021-02-24)
Pregnancy is a complicated physiological process. The multifaceted regulation of maternal-fetal interface is of great importance for maintaining normal pregnancy and avoiding fetal rejection and secondary abortion. Previous studies have focused on the clinical features or pathological biomarkers of fetal
View All Related Papers

Global Trade Item Number

SKUGTIN
P7828-100ML04061834398219
P7828-25ML04061834398233
P7828-1L04061834398226
P7828-500ML04061834398240

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