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P8045

Protein G-Biotin from Streptococcus sp.

recombinant, expressed in unspecified host, lyophilized powder

Synonym(s):

Biotinylated Protein G, Protein G Biotin, Protein G-Biotin

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1 mg

Available to ship TODAYfromMILWAUKEE

$447.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00132111
Form:
lyophilized powder
Recombinant:
expressed in unspecified host

$447.00

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recombinant

expressed in unspecified host

Quality Segment

200

conjugate

biotin conjugate

form

lyophilized powder

composition

Protein, ~90% biuret

extent of labeling

2-4 mol d-biotin per mol protein

technique(s)

direct ELISA: 1:70,000 using human IgG

storage temp.

2-8°C

General description

Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein.
Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.

Application

Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities.
Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.

Biochem/physiol Actions

Binds IgG only from most mammalian species except cat; binds chicken IgG.

Packaging

Package size based on protein content

Preparation Note

Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.

Other Notes

Truncated - lacks albumin, Fab and membrane binding sites.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Protein G-Biotin from Streptococcus sp. recombinant, expressed in unspecified host, lyophilized powder

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Sigma-Aldrich

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Protein L from Peptostreptococcus magnus >95% (SDS-PAGE), recombinant, expressed in E. coli, essentially salt-free, lyophilized powder

Sigma-Aldrich

P3101

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Protein A–Peroxidase from Staphylococcus aureus/horseradish lyophilized powder

Sigma-Aldrich

P8651

Protein A–Peroxidase from Staphylococcus aureus/horseradish

description

recombinant, expressed in unspecified host, lyophilized powder

description

recombinant, expressed in unspecified host, lyophilized powder

description

>95% (SDS-PAGE), recombinant, expressed in E. coli, essentially salt-free, lyophilized powder

description

lyophilized powder

recombinant

expressed in unspecified host

recombinant

expressed in unspecified host

recombinant

expressed in E. coli

recombinant

-

technique(s)

direct ELISA: 1:70,000 using human IgG

technique(s)

direct ELISA: 1:100,000 using human IgG

technique(s)

indirect ELISA: 1:100,000

technique(s)

direct ELISA: 1:80,000 using human IgG

form

lyophilized powder

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200, 100

conjugate

biotin conjugate

conjugate

peroxidase conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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