The exact temperature range for RNase inhibition has not been determined. This product has shown activity from room temperature to 50 °C. Denaturing conditions, such as urea or temperatures above 50 °C, should be avoided as they may cause the release of active ribonuclease from the complex.
R2520
Ribonuclease inhibitor human
Inhibits ribonucleases during transcription and translation experiments
Synonym(s):
RNase Inhibitor, Ribonuclease Inhibitor
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| Size/SKU | Availability | Price |
|---|---|---|
2500 units | Check Cart for Availability | $224.00 |
10000 units | Check Cart for Availability | $717.00 |
20000 units | Check Cart for Availability | $1,360.00 |
About This Item
$224.00
Check Cart for Availability
biological source
human placenta
Quality Segment
grade
Molecular Biology
form
buffered aqueous solution
mol wt
~50 kDa
concentration
30,000-50,000 units/mL
shipped in
dry ice
storage temp.
−20°C
General description
Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is human placenta. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes.
Application
Suitable for use in
- in vitro inhibition of ribonucleases
- cDNA synthesis
- RT-PCR
- in vitro transcription and translation
Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.
Biochem/physiol Actions
Inhibits RNase by forming a tight, non-covalent 1:1 complex.
Inhibits RNase by forming a tight, non-covalent 1:1 complex. Also inhibits angiogenin, a neovascularization promoter which is homologous with RNase A.
Physical form
Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT
Other Notes
One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.
Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.
Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT
This inhibitor of RNase activity is isolated from human placenta and supplied as a buffered aqueous glycerol solution.
1 of 1
This Item | |||
|---|---|---|---|
| description Inhibits ribonucleases during transcription and translation experiments | description recombinant, expressed in E. coli, 20-40 units/μL | description from Escherichia coli H 560 pol A1 | description Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein |
| biological source human placenta | biological source - | biological source Escherichia coli ( H 560 pol A1) | biological source bovine pancreas |
| Quality Level 200 | Quality Level 300 | Quality Level - | Quality Level 200 |
| form buffered aqueous solution | form buffered aqueous solution | form solution | form buffered aqueous solution |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C (−15°C to −25°C) | storage temp. −20°C |
| mol wt ~50 kDa | mol wt ~50 kDa | mol wt - | mol wt ~13,700 |
Storage Class
12 - Non Combustible Liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Hi, what is the temperature range in which this product inhibits RNase activity? Thanks,
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