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Ribonuclease A from bovine pancreas

Name: Ribonuclease A from bovine pancreas
Brand: SIGMA
Price: 142 USD
Availability: InStock

Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein

Synonym(s):

Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

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Size/SKU	Availability	Price
100 mg	Available to ship TODAYfromMILWAUKEE	$142.00
250 mg	Available to ship TODAYfromMILWAUKEE	$283.00
1 g	Available to ship TODAYfromMILWAUKEE	$932.00

About This Item

CAS Number:

9001-99-4

NACRES:

NA.54

UNSPSC Code:

12352204

EC Number:

232-646-6

MDL number:

MFCD00132181

EC Number:

3.1.27.5 (BRENDA, IUBMB)

Specific activity:

>= 60 Kunitz units/mg protein

Biological source:

bovine pancreas

Concentration:

≥60% (SDS-PAGE)

$142.00

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Properties

biological source

bovine pancreas

Quality Segment

300

type

Type II-A

form

solid

specific activity

>= 60 Kunitz units/mg protein

mol wt

~13,700

concentration

≥60% (SDS-PAGE)

technique(s)

cell based assay: suitable

impurities

salt, essentially free

input

general use sample(s)

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

SMILES string

[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

InChI key

CQOVPNPJLQNMDC-UHFFFAOYSA-N

Description

General description

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds.

Application

RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
RNase A is also used in RNA sequence analysis and protection assays.
RNase A has been used as a tool for computer-aided drug design.
RNase A supports the analysis of RNA sequences.
RNase A hydrolyze RNA contained in protein samples.
Purification of DNA is supported by RNase A.

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease A from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography.

Features and Benefits

Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.

Preparation Note

Chromatographically purified

Analysis Note

Protein determined by E.

RNase A purity determined by SDS-PAGE

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This Item	R5500	R4642	R4875
Sigma-Aldrich R5000 Ribonuclease A from bovine pancreas	Sigma-Aldrich R5500 Ribonuclease A from bovine pancreas	Sigma-Aldrich R4642 Ribonuclease A from bovine pancreas	Sigma-Aldrich R4875 Ribonuclease A from bovine pancreas
description Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein	description Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein	description (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)	description Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein
biological source bovine pancreas	biological source bovine pancreas	biological source bovine pancreas	biological source bovine pancreas
specific activity >= 60 Kunitz units/mg protein	specific activity 75-125 Kunitz units/mg protein	specific activity -	specific activity ≥50 Kunitz units/mg protein
technique(s) cell based assay: suitable	technique(s) cell based assay: suitable	technique(s) -	technique(s) cell based assay: suitable
form solid	form lyophilized powder	form (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)	form powder
concentration ≥60% (SDS-PAGE)	concentration -	concentration 20-40 mg/mL	concentration ≥60% (RNase A, SDS-PAGE)
application(s) diagnostic assay manufacturing	application(s) diagnostic assay manufacturing	application(s) -	application(s) diagnostic assay manufacturing

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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