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Ribonuclease A from bovine pancreas

Name: Ribonuclease A from bovine pancreas
Brand: SIGMA
Price: 336 USD
Availability: InStock

Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein

Synonym(s):

Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

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Size/SKU	Availability	Price
100 mg	Estimated to ship onOctober 01, 2026fromMILWAUKEE	$336.00
250 mg	Estimated to ship onOctober 01, 2026fromMILWAUKEE	$680.00
500 mg	Available to ship TODAYfromMILWAUKEE	$1,220.00
1 g	Available to ship TODAYfromMILWAUKEE	$2,040.00 $1,734.00

About This Item

CAS Number:

9001-99-4

NACRES:

NA.54

UNSPSC Code:

12352204

MDL number:

MFCD00132181

EC Number:

3.1.27.5 (BRENDA, IUBMB)

Specific activity:

≥70 Kunitz units/mg protein

Assay:

≥90% (SDS-PAGE)

Biological source:

bovine pancreas

$336.00

Estimated to ship onOctober 01, 2026Details

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Properties

biological source

bovine pancreas

Quality Segment

200

type

Type X-A

assay

≥90% (SDS-PAGE)

form

buffered aqueous solution

specific activity

≥70 Kunitz units/mg protein

mol wt

~13,700

input

general use sample(s)

foreign activity

protease, essentially free

storage temp.

−20°C

SMILES string

[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

InChI key

CQOVPNPJLQNMDC-UHFFFAOYSA-N

Description

General description

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. The ribonuclease (RNase) gene is mapped to human chromosome 14.

Application

RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
RNase A is also used in RNA sequence analysis and protection assays.
RNase A has been used as a tool for computer-aided drug design.
RNase A supports the analysis of RNA sequences.
RNase A hydrolyze RNA contained in protein samples.
Purification of DNA is supported by RNase A.

Ribonuclease A from bovine pancreas has been used to treat human glioblastoma U251 cell line prior to harvesting, for flow cytometry analysis, T suppressor cells and embryonic stem cell chromatin.

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the mechanism of heavy metal ions on RNase activity. Ribonuclease A from bovine pancreas has also been used in a study to investigate the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins.

Biochem/physiol Actions

The structure of Ribonuclease A (RNase A) comprises of four disulfide and catalytic triad of two histidines crucial for its functionality. RNase A displays three-dimensional domain swapping of the α-helical N-terminal and the C-terminal β-strand. It can exist as dimer or oligomers. The level of RNase is elevated in cancers and infectious diseases. RNase A family of enzymes serve as potential drug targets. The homolog and variants of RNase A are potential chemotherapeutic agents.

Features and Benefits

Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.

Physical form

Solution in 0.2 M sodium phosphate buffer, pH 6.4

Preparation Note

A further chromatographic purification of R5503 to yield essentially pure RNAse A

Analysis Note

Protein determined by E.

RNase A purity determined by SDS-PAGE

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Show Differences

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This Item	R5500	R5125	R6513
Sigma-Aldrich R5250 Ribonuclease A from bovine pancreas	Sigma-Aldrich R5500 Ribonuclease A from bovine pancreas	Sigma-Aldrich R5125 Ribonuclease A from bovine pancreas	Sigma-Aldrich R6513 Ribonuclease A from bovine pancreas
description Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein	description Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein	description Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein	description Molecular Biology, ≥70 Kunitz units/mg protein, lyophilized
biological source bovine pancreas	biological source bovine pancreas	biological source bovine pancreas	biological source -
assay ≥90% (SDS-PAGE)	assay ≥90% (SDS-PAGE)	assay ≥85% RNase A basis (SDS-PAGE)	assay -
specific activity ≥70 Kunitz units/mg protein	specific activity 75-125 Kunitz units/mg protein	specific activity 85-140 Kunitz units/mg protein	specific activity ≥70 Kunitz units/mg protein
form buffered aqueous solution	form lyophilized powder	form lyophilized powder	form lyophilized
mol wt ~13,700	mol wt ~13,700	mol wt ~13,700	mol wt 13.7 kDa, ~13,700
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

WGK

WGK 2

PPE (personal protective equipment)

Eyeshields, Gloves

Flash Point (°F)

Not applicable

Flash Point (°C)