This product is not tested for DNase activity.
R5503
Ribonuclease A from bovine pancreas
Type I-AS, 50-100 Kunitz units/mg protein
Synonym(s):
Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
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| Size/SKU | Availability | Price |
|---|---|---|
100 mg | Available to ship TODAYfromMILWAUKEE | $131.00 |
500 mg | Available to ship TODAYfromMILWAUKEE | $394.00 |
1 g | Available to ship TODAYfromMILWAUKEE | $675.00 |
5 g | Available to ship TODAYfromMILWAUKEE | $2,450.00 |
10 g | Available to ship TODAYfromMILWAUKEE | $3,910.00 |
About This Item
CAS Number:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-646-6
MDL number:
Specific activity:
50-100 Kunitz units/mg protein
Biological source:
bovine pancreas
biological source
bovine pancreas
Quality Segment
type
Type I-AS
form
lyophilized powder
specific activity
50-100 Kunitz units/mg protein
mol wt
~13,700
technique(s)
cell based assay: suitable
impurities
salt, essentially free
input
general use sample(s)
suitability
suitable for mRNA or total RNA extracted from cells and tissues
application(s)
diagnostic assay manufacturing
foreign activity
protease, essentially free
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
General description
RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.
Application
- RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
- RNase A is also used in RNA sequence analysis and protection assays.
- RNase A has been used as a tool for computer-aided drug design.
- RNase A supports the analysis of RNA sequences.
- RNase A hydrolyze RNA contained in protein samples.
- Purification of DNA is supported by RNase A.
Biochem/physiol Actions
Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.
Features and Benefits
Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.
Preparation Note
Salt fractionated and chromatographically purified.
Analysis Note
Protein determined by E.
1 of 1
This Item | |||
|---|---|---|---|
| description Type I-AS, 50-100 Kunitz units/mg protein | description Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein | description Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein | description Proteomics Grade |
| specific activity 50-100 Kunitz units/mg protein | specific activity 75-125 Kunitz units/mg protein | specific activity 85-140 Kunitz units/mg protein | specific activity - |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) - |
| biological source bovine pancreas | biological source bovine pancreas | biological source bovine pancreas | biological source - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - |
| suitability suitable for mRNA or total RNA extracted from cells and tissues | suitability suitable for mRNA or total RNA extracted from cells and tissues | suitability suitable for molecular biology | suitability - |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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