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R5506

Anti-Rabbit IgG (whole molecule) antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Rabbit IgG Antibody

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2 mL

Available to ship TODAYfromMILWAUKEE

$245.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162781
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA (i), QPA
Citations:
22

$245.00

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biological source

goat

Quality Segment

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

indirect ELISA: 1:30,000, quantitative precipitin assay: 3.0-4.5 mg/mL

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Immunoglobulins (Igs) belong to the immunoglobulin super-family and have two heavy (H) and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region. IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4. The IgG heavy chain region is mapped to human chromosome 14.
Rabbit immunoglobulins (IgGs) are often used as primary antibodies in several protein assays. Thus, anti-rabbit IgGs can be used as secondary antibodies to study and characterize target proteins bound to rabbit IgGs. Anti-Rabbit IgG (whole molecule) antibody is specific for IgGs in rabbits.

Immunogen

Rabbit IgG

Application

Anti-Rabbit IgG (whole molecule) antibody is suitable for use in quantitative precipitin assay, immunoelectrophoresis, immunohistochemistry , and laser scattering microscopy .
Anti-Rabbit IgG (whole molecule) antibody produced in goat has been used as secondary antibody in vivo xenograft assay in non-small-cell lung cancer (NSCLC) tissues and in western blotting detection in endometrial sample from cattle.

Biochem/physiol Actions

Anti-Rabbit IgG (whole molecule) antibody is specific for IgGs in rabbits.
Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG produces fragment crystallizable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies. IgG1 class is the most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions, and IgG4 is associated with asymptomatic infection.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as preservative

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Rabbit IgG (whole molecule) antibody produced in goat IgG fraction of antiserum, buffered aqueous solution

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R5130

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description

IgG fraction of antiserum, buffered aqueous solution

description

affinity isolated antibody, lyophilized powder

description

affinity isolated antibody, lyophilized powder

description

affinity isolated antibody, lyophilized powder

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

quantitative precipitin assay: 3.0-4.5 mg/mL, indirect ELISA: 1:30,000

technique(s)

immunoelectrophoresis: suitable

technique(s)

immunoelectrophoresis: suitable

technique(s)

quantitative precipitin assay: suitable

biological source

goat

biological source

goat

biological source

goat

biological source

goat

form

buffered aqueous solution

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

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Storage Class

10 - Combustible liquids

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