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RAB0278

Rat IL-1 β ELISA Kit

for cell and tissue lysates

Synonym(s):

Il-1 beta, Interleukin-1 beta

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1 kit
Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
$608.00

About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
rat ... Il1b(24494)
Input:
sample type cell lysate
sample type tissue lysate
Species reactivity:
rat
Storage temp.:
−20°C
Shipped in:
wet ice

$608.00


Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)

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species reactivity

rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 68.59-50000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... Il1b(24494)

General description

The Rat IL-1 β ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1β cell lysate and tissue lysate.

Immunogen

Recombinant Rat IL-1 β

Application

For research use only. Not for use in diagnostic procedures

Rat IL-1 Β ELISA Kit has been used to measure IL-1 Β levels .
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Pro-inflammatory cytokine IL-1 Β (IL1B), which is highly expressed on Β-adrenoreceptor in obese adipose tissues, is implicated in induction of uncoupling protein 1 (UCP1) expression via extracellular signal-regulated kinase (ERK) activation in adipocytes. Macrophage autophagy inhibits the production of inflammasome-dependent IL-1Β and thereby it plays a vital role in reducing acute toxin-induced liver injury and death. In rats, IL-1Β enhanced neurogenesis and plasticity of carotid bodies (CB) by triggering ERK1/2 activation. IL-1Β plays a vital role in alteration of glomerular hemodynamics via stimulating the production of prostaglandin E2 (PGE2) in the mesangial cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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This Item
RAB0272RAB0302RAB0312
description

for cell and tissue lysates

description

for serum, plasma and cell culture supernatant

description

for cell and tissue lysates

description

for for cell lysate and tissue lysate

Gene Information

rat ... Il1b(24494)

Gene Information

rat ... Il1a(24493)

Gene Information

rat ... Il4(287287)

Gene Information

rat ... Il6(24498)

species reactivity

rat

species reactivity

rat

species reactivity

rat

species reactivity

rat

input

sample type cell lysate
sample type tissue lysate

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type cell lysate
sample type tissue lysate

input

sample type tissue lysate
sample type cell lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 68.59-50000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 10.97-8000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1.5 pg/mL
standard curve range: 0.66-160 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 40.96-10000 pg/mL

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


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Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA 5X Sample Diluent Buffer (Item D2)
    SDS

  • ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
    SDS

  • 2X Cell Lysis Buffer (Item J)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids



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