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RAB0369

Human MMP-7 ELISA Kit

on for cell culture supernatant

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1 kit
Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
$608.00

About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... MMP7(4316)
Input:
sample type cell culture supernatant(s)
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice

$608.00


Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)

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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 150 pg/mL
standard curve range: 0.137-100 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MMP7(4316)

General description

The human MMP-7 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-7 in cell culture supernatants. Matrix metalloproteinase-7 (MMP-7)/matrilysin is a small proteolytic enzyme. It secretes zinc- and calcium-dependent endopeptidase. It is expressed in normal endometrial, bronchial, ductal, skin glandular epithelial, urogenital, and gastrointestinal tissues. MMP-7 plays a key role in cell proliferation, migration, invasion, and apoptosis. Elevated expression of MMP-7 is seen in several cancers, such as esophageal, gastric, gallbladder, pancreatic, colorectal, bladder, ovarian, breast, lung, and melanoma.

Immunogen

Recombinant Human MMP7

Application

Human MMP-7 ELISA Kit has been used in ELISA to measure the recovery of the cancer biomarkers/MMP-7 in isothermally vitrified samples. It has also been used in ELISA to determine MMP7 concentrations in multiple myeloma (MM) cell culture supernatants.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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RAB0370RAB0367RAB0373
description

on for cell culture supernatant

description

for serum, plasma, conditioned medium, urine

description

for serum, plasma, cell culture supernatant and urine

description

for serum, plasma and cell culture supernatant

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

mouse

Gene Information

human ... MMP7(4316)

Gene Information

human ... MMP8(4317)

Gene Information

human ... MMP3(185250)

Gene Information

mouse ... Mmp9(17395)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 150 pg/mL
standard curve range: 0.137-100 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 8.23-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 300 pg/mL
standard curve range: 0.41-100 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 74.07-18000 pg/mL

input

sample type cell culture supernatant(s)

input

sample type plasma
sample type serum
sample type urine
sample type conditioned medium

input

sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Certificates of Analysis (COA)

Lot/Batch Number

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Questions

  1. Is the MMP7 a total form or an active form in this kit?

    1 answer
    1. The capture antibody used in this kit will recognize both the pro and active form of MMP-7. The immunogen is human recombinant MMP-7, aa18 - 267. The standard provided in the kit is the pro form,

      Response from Ray Bio - not to be included in response
      Capture Ab: Mouse Monoclonal IgG2B
      Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP 7
      aa18-aa267, accession# NP_002414.1
      Specificity: detects human MMP-7 in pro and active forms (seen in WB)

      Detection Ab: Polyclonal Goat IgG
      Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP 7
      aa18-aa267, accession # P09237

      Additionally, the standard used is recombinant Human MMP-7 in pro form (aa18-267).

      Helpful?

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