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RAB0397

Human PDGF-BB ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

PDGF subunit B, PDGF-2, Platelet-derived growth factor subunit B

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1 kit
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$770.00

About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... PDGFB(5155)
Input:
sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice

$770.00


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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.549-400 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PDGFB(5155)

General description

The Human PDGF-BB ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PDGF-BB in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human PDGF-BB

Application

For research use only. Not for use in diagnostic procedures.
Human PDGF-BB ELISA Kit has been used to measure PDGF-BB (platelet-derived growth factor BB) concentration in human coronary artery smooth muscle cells (HCASMCs).

Biochem/physiol Actions

PDGF-BB (platelet-derived growth factor BB) stimulates lymphatic metastasis and intratumoral lymphangiogenesis. PDGF-BB might be implicated in regulation of angiogenesis and endothelial proliferation. PDGF-BB expressed in placental endothelial cell activates signal transducer and activator of transcription 3 (STAT3) pathway and recruits human placental multipotent mesenchymal stromal cells (hPMSCs) for placental vascularization. Heterozygous mutation in PDGF-B is associated with primary familial brain calcification.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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RAB0396RAB0400RAB0394
Gene Information

human ... PDGFB(5155)

Gene Information

human ... PDGFA(5154), PDGFB(5155)

Gene Information

human ... PDGFRB(5159)

Gene Information

human ... PDGFA(5154)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

input

-

input

sample type urine
sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type plasma
sample type serum
sample type urine
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.549-400 pg/mL

assay range

-

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 24.69-18000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 40 pg/mL
standard curve range: 41.15-30000 pg/mL

detection method

colorimetric

detection method

-

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable


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Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
    SDS

  • ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids



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Related Content


PDGF-BB induces intratumoral lymphangiogenesis and promotes lymphatic metastasis.
Cao R
Cancer Cell, 6(4), 333-345 (2004)
Role of Myoendothelial Gap Junctions in the Regulation of Human Coronary Artery Smooth Muscle Cell Differentiation by Laminar Shear Stress.
Zhang Z
Cellular Physiology and Biochemistry, 39(2), 423-437 (2016)
Callie Frey et al.
Platelets, 31(7), 952-959 (2020-01-15)
Patients with heart failure (HF) and left ventricular assist devices (LVAD) have dysregulated thrombo-inflammatory responses, mediated in part by platelets. While studies of platelet activation have been undertaken in HF, changes in the platelet transcriptome in HF patients following mechanical



Global Trade Item Number

SKUGTIN
RAB0397-1KT04061834962250

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