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Human PDGF-BB ELISA Kit

Name: Human PDGF-BB ELISA Kit
Brand: SIGMA
Price: 608 USD

for serum, plasma, cell culture supernatant and urine

Synonym(s):

PDGF subunit B, PDGF-2, Platelet-derived growth factor subunit B

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Pack Size	SKU	Availability	Price
1 kit		Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)	$608.00

About This Item

NACRES:

NA.32

UNSPSC Code:

41116158

Gene information:

human ... PDGFB(5155)

Input:

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

Species reactivity:

human

Storage temp.:

−20°C

Shipped in:

wet ice

$608.00

Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)

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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.549-400 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PDGFB(5155)

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Protein Biology

Enzyme-Linked Immunosorbent Assay (ELISA) Kits

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Show Differences

1 of 1

This Item	RAB0398	RAB0396	RAB0399
Sigma-Aldrich RAB0397 Human PDGF-BB ELISA Kit	Sigma-Aldrich RAB0398 Human PDGF-BB ELISA Kit	Sigma-Aldrich RAB0396 Human PDGF-AB ELISA Kit	Sigma-Aldrich RAB0399 Human PDGF R α ELISA Kit
Gene Information human ... PDGFB(5155)	Gene Information human ... PDGFB(5155)	Gene Information human ... PDGFA(5154), PDGFB(5155)	Gene Information human ... PDGFRA(5156)
species reactivity human	species reactivity human	species reactivity human	species reactivity human
input sample type serum sample type plasma sample type cell culture supernatant(s) sample type urine	input sample type tissue lysate sample type cell lysate	input -	input sample type cell culture supernatant(s) sample type serum sample type plasma sample type urine
assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 1 pg/mL standard curve range: 0.549-400 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 1 pg/mL standard curve range: 0.549-400 pg/mL	assay range -	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 400 pg/mL standard curve range: 0.49-120 ng/mL
technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

General description

The Human PDGF-BB ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PDGF-BB in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human PDGF-BB

Application

For research use only. Not for use in diagnostic procedures.
Human PDGF-BB ELISA Kit has been used to measure PDGF-BB (platelet-derived growth factor BB) concentration in human coronary artery smooth muscle cells (HCASMCs).

Biochem/physiol Actions

PDGF-BB (platelet-derived growth factor BB) stimulates lymphatic metastasis and intratumoral lymphangiogenesis. PDGF-BB might be implicated in regulation of angiogenesis and endothelial proliferation. PDGF-BB expressed in placental endothelial cell activates signal transducer and activator of transcription 3 (STAT3) pathway and recruits human placental multipotent mesenchymal stromal cells (hPMSCs) for placental vascularization. Heterozygous mutation in PDGF-B is associated with primary familial brain calcification.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.

Description

SDS

RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

RABSTOP3ELISA Stop Solution (Item I)SDS

RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

RABWASH420X Wash Buffer (Item B)SDS

pictograms

GHS05

signalword

Warning

hcodes

H290

pcodes

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids

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PDGF-BB induces intratumoral lymphangiogenesis and promotes lymphatic metastasis.

Cao R

Cancer Cell, 6(4), 333-345 (2004)

Role of Myoendothelial Gap Junctions in the Regulation of Human Coronary Artery Smooth Muscle Cell Differentiation by Laminar Shear Stress.

Zhang Z

Cellular Physiology and Biochemistry, 39(2), 423-437 (2016)

Longitudinal assessment of the platelet transcriptome in advanced heart failure patients following mechanical unloading.

Callie Frey et al.

Platelets, 31(7), 952-959 (2020-01-15)

Patients with heart failure (HF) and left ventricular assist devices (LVAD) have dysregulated thrombo-inflammatory responses, mediated in part by platelets. While studies of platelet activation have been undertaken in HF, changes in the platelet transcriptome in HF patients following mechanical

PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.

Battegay EJ

The Journal of Cell Biology, 125(4), 917-928 (1994)

Adenosine deaminase 2 produced by infiltrative monocytes promotes liver fibrosis in nonalcoholic fatty liver disease.

Shilpa Tiwari-Heckler et al.

Cell reports, 37(4), 109897-109897 (2021-10-28)

Elevated circulating activity of adenosine deaminase 2 (ADA2) is associated with liver fibrosis in nonalcoholic fatty liver disease (NAFLD). In the liver of NAFLD patients, ADA2-positive portal macrophages are significantly associated with the degree of liver fibrosis. These liver macrophages

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Global Trade Item Number

SKU	GTIN
RAB0397-1KT	04061834962250

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