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Human Osteoprotegerin / TNFRSF11b ELISA Kit

Name: Human Osteoprotegerin / TNFRSF11b ELISA Kit
Brand: SIGMA
Price: 608 USD

for serum, plasma, cell culture supernatant and urine

Synonym(s):

OPG

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Pack Size	SKU	Availability	Price
1 kit		Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)	$608.00

About This Item

NACRES:

NA.32

UNSPSC Code:

41116158

Gene information:

human ... TNFRSF11B(4982)

Input:

sample type serum
sample type urine
sample type cell culture supernatant(s)
sample type plasma

Species reactivity:

human

Storage temp.:

−20°C

Shipped in:

wet ice

$608.00

Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)

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Properties

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type serum
sample type urine
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 1.23-900 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TNFRSF11B(4982)

Description

General description

The Human Osteoprotegerin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Osteoprotegerin in serum, plasma, cell culture supernatants and urine.

Osteoprotegerin (OPG) is encoded by the gene mapped to human chromosome 8q24. It is a member of the TNFR (tumor necrosis factor receptor) superfamily that can act as a decoy receptor for RANKL (receptor activator of nuclear factor kappa-B ligand) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). OPG is expressed in a wide variety of tissues, including adult heart, lung, kidney and liver. It is secreted both as a monomeric and a dimeric protein.

Immunogen

Recombinant Human OPG

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Binding of soluble osteoprotegerin (OPG) to sRANKL (soluble receptor activator of nuclear factor κ-B ligand) inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells. This process leads to excess accumulation of bone and cartilage. Changes in the level of serum OPG are associated with atherosclerosis and cardiovascular disorders(CVDs). It might also be associated with malignancy and plays a regulatory role in multiple myeloma, prostate, breast, bladder and gastric carcinoma. OPG plays a vital role in bone metabolism and is involved in inhibition of ovariectomy-associated bone loss in rats. It is also used as a potent biomarker for CVD.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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This Item	RAB0436	RAB0493	RAB0550
Sigma-Aldrich RAB0484 Human Osteoprotegerin / TNFRSF11b ELISA Kit	Sigma-Aldrich RAB0436 Human Osteopontin ELISA Kit	Sigma-Aldrich RAB0493 Mouse Osteoprotegerin ELISA Kit	Sigma-Aldrich RAB0550 Human TNFRSF11A ELISA Kit
species reactivity human	species reactivity human	species reactivity mouse	species reactivity human
Gene Information human ... TNFRSF11B(4982)	Gene Information human ... SPP1(6696)	Gene Information mouse ... Tnfrsf11b(18383)	Gene Information human ... TNFRSF11A(8792)
input sample type serum sample type urine sample type cell culture supernatant(s) sample type plasma	input sample type serum sample type plasma sample type urine sample type cell culture supernatant(s)	input sample type plasma sample type serum sample type cell culture supernatant(s)	input sample type urine sample type cell culture supernatant(s) sample type serum sample type plasma
assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 1 pg/mL standard curve range: 1.23-900 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 50 pg/mL standard curve range: 74.07-18000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 1 pg/mL standard curve range: 1.65-1200 pg/mL	assay range inter-assay cv: <10% intra-assay cv: <12% sensitivity: 0.4 ng/mL
detection method colorimetric	detection method colorimetric	detection method colorimetric	detection method colorimetric
technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable