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S3917

Single-strand Binding Protein from Escherichia coli

>95% (SDS-PAGE), Molecular Biology, Single strand binding protein to enhance PCR specificity

Synonym(s):

SSB

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0.1 mg
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$182.00
0.5 mg
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$630.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.52
MDL number:
MFCD00213047
Form:
liquid
Assay:
>95% (SDS-PAGE)

$182.00

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grade

Molecular Biology

Quality Segment

assay

>95% (SDS-PAGE)

form

liquid

technique(s)

whole genome amplification: suitable

color

colorless

foreign activity

DNase and RNase, none detected

shipped in

wet ice

storage temp.

−20°C

General description

Single-strand Binding Protein is a homotetramer, found in E. coli during the event of DNA metabolism.

Application

Single-strand Binding Protein from Escherichia coli has been used in in vitro RNA/Single-Strand Binding Protein (SSB) complex formation assays and in protein purification to determine integrity and functionality of the integron attC sites.
Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.

Biochem/physiol Actions

Single-strand Binding Protein (SSBP) exhibits high affinity towards single stranded DNA important for DNA metabolic processes. Single-stranded DNA-binding protein hSSB1 is critical for genomic stability and assists the delivery of primers during the formation of lagging standard. It also prevents the formation of inhibitory secondary structures in the exposed lagging-strand of DNA during replication. SSBP mediates the recruitment of other proteins to their sites of function on DNA.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol

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Single-strand Binding Protein from Escherichia coli >95% (SDS-PAGE), Molecular Biology, Single strand binding protein to enhance PCR specificity

Sigma-Aldrich

S3917

Single-strand Binding Protein from Escherichia coli

Fpg Protein from Escherichia coli ≥90% (SDS-PAGE), buffered aqueous glycerol solution, >20,000 units/mg protein, suitable for genomic analysis

Sigma-Aldrich

F3174

Fpg Protein from Escherichia coli

Thioredoxin from Escherichia coli recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

Sigma-Aldrich

T0910

Thioredoxin from Escherichia coli

DNA Gyrase from Escherichia coli aqueous glycerol solution

Sigma-Aldrich

D0690

DNA Gyrase from Escherichia coli

description

>95% (SDS-PAGE), Molecular Biology, Single strand binding protein to enhance PCR specificity

description

≥90% (SDS-PAGE), buffered aqueous glycerol solution, >20,000 units/mg protein, suitable for genomic analysis

description

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

description

aqueous glycerol solution

technique(s)

whole genome amplification: suitable

technique(s)

nucleic acid detection: suitable

technique(s)

activity assay: suitable

technique(s)

cell based assay: suitable

grade

Molecular Biology

grade

-

grade

-

grade

-

assay

>95% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

-

form

liquid

form

buffered aqueous glycerol solution

form

essentially salt-free, lyophilized powder

form

aqueous glycerol solution

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Danger

Hazard Codes

H318,H410,EUH430

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1

Storage Class

10 - Combustible liquids

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