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Anti-Sirt1 antibody, Mouse monoclonal

Name: Anti-Sirt1 antibody, Mouse monoclonal
Brand: SIGMA
Price: 730 USD
Availability: InStock

clone SIR11, purified from hybridoma cell culture

Synonym(s):

Anti-SIRTUIN1, Anti-Sir2α, Anti-mouse homologue of yeast Sir2

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Size/SKU	Availability	Price
200 μL	Check Cart for Availability	$730.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD08705602

Conjugate:

unconjugated

Clone:

SIR11, monoclonal

Application:

ARR, ELISA (i), WB

Citations:

$730.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture, purified immunoglobulin

antibody product type

primary antibodies

clone

SIR11, monoclonal

form

buffered aqueous solution

mol wt

antigen ~105 kDa

species reactivity

mouse

concentration

~2 mg/mL

technique(s)

indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using total cell extract of C-2 cells

isotype

IgG1

UniProt accession no.

Q923E4

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Sirt1(93759)

Description

General description

Monoclonal Anti-Sirt1 (Sir2) (mouse IgG1 isotype) is derived from the hybridoma SIR11 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids at the C-terminus of mouse Sir2 with C-terminal added lysine. Sirt proteins are a family of proteins that are found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity. Sirtuin 1 (SIRT1) protein, a mammalian homolog of yeast Sir2, is a NAD-dependent histone deacetylase.

Application

Anti-Sirt1 antibody, Mouse monoclonal has been used in:

western blot analysis
enzyme-linked immunosorbent assay (ELISA)
immunohistochemical methods

Monoclonal Anti-Sirt1 antibody is suitable for use in western blot (1-2 μg/mL using total cell extract of C-2 cells), immunoblot (approx. 105 kDa), microarray and indirect ELISA.

Biochem/physiol Actions

Monoclonal Anti-Sirt1 (Sir2α) antibody is specific for Sir2α in mice.

SIRT1 is a highly conserved sirtuin family deactelyase that modulates heart metabolism . SIRT1 has also been implicated in senescence and aging . Thus, SIRT1 may be used as a target for treating age-related diseases.

Sirt1 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes O-acetyl-ADP-ribose. Through histone deacetylation, Sirt1 may silence chromatin. It appears that Sirt1 NAD requirement makes this protein an important player in the pathway that leads to increased life span of several species through calorie restriction. Sirtuin 1 (SIRT1) plays an important role in neuronal proliferation and vascular cells. It also plays an athero-protective role. Mutations in the SIRT1 gene affects the metabolism, cholesterol and fat deposition. SIRT1 has the ability to intermediate the insulin resistance through an inflammatory process, reactive oxygen species, gluconeogenesis and adiponectin level.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	S5313	S5947	S8447
Sigma-Aldrich S5196 Anti-Sirt1 antibody, Mouse monoclonal	Sigma-Aldrich S5313 Anti-Sir2 (AS-16) antibody produced in rabbit	Sigma-Aldrich S5947 Anti-Sirt7 antibody produced in rabbit	Sigma-Aldrich S8447 Anti-Sirt2 antibody produced in rabbit
description clone SIR11, purified from hybridoma cell culture	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
clone SIR11, monoclonal	clone polyclonal	clone polyclonal	clone polyclonal
species reactivity mouse	species reactivity mouse	species reactivity mouse (predicted), human	species reactivity rat, mouse, human
Gene Information mouse ... Sirt1(93759)	Gene Information mouse ... Sirt1(93759)	Gene Information human ... SIRT7(51547) mouse ... Sirt7(209011)	Gene Information human ... SIRT2(22933) mouse ... Sirt2(64383) rat ... Sirt2(361532)
antibody form purified from hybridoma cell culture, purified immunoglobulin	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
biological source mouse	biological source rabbit	biological source rabbit	biological source rabbit

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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