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Superoxide Dismutase from bovine erythrocytes

Name: Superoxide Dismutase from bovine erythrocytes
Brand: SIGMA
Price: 122 USD

≥3,000 units/mg protein, BioReagent, lyophilized powder, suitable for cell culture

Synonym(s):

SOD, Superoxide: superoxide oxidoreductase

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Size/SKU	Availability	Price
15000 units	Check Cart for Availability	$122.00
30000 units	Check Cart for Availability	$179.00
75000 units	Check Cart for Availability	$353.00

About This Item

CAS Number:

9054-89-1

UNSPSC Code:

12352204

NACRES:

NA.75

EC Number:

232-943-0

MDL number:

MFCD00132404

EC Number:

1.15.1.1 (BRENDA, IUBMB)

Specific activity:

≥3,000 units/mg protein

Biological source:

bovine erythrocytes

$122.00

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Properties

biological source

bovine erythrocytes

Quality Segment

200

product line

BioReagent

form

lyophilized powder

specific activity

≥3,000 units/mg protein

mol wt

32.5 kDa

packaging

pkg of 15000 units

technique(s)

cell culture | mammalian: suitable

pH

7.6-10.5

shipped in

dry ice

storage temp.

−20°C

Description

General description

Superoxide Dismutase from bovine erythrocytes is a metalloprotein which disproportionates superoxide anion radicals. It is a 31.5 kDa copper binding protein and displays a conserved domain and fold. It is a homodimer with one copper and zinc ion per subunit and has antiparallel “greek-key” β barrel fold.

Application

Superoxide Dismutase (SOD) from bovine erythrocytes has been used:

for measuring the superoxide radical using the electron paramagnetic resonance spin in human brain microvascular endothelial cells
for measuring superoxide production in cytochrome C assay in peripheral blood mononuclear cells
as a standard in characterization of hen egg SOD using Fourier-transform infrared spectroscopy (FTIR) and matrix-assisted laser desorption/ionization (MALDI) analysis

Biochem/physiol Actions

Superoxide Dismutase from bovine erythrocytes catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It serves as an antioxidant and plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Analysis Note

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Other Notes

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀of 0.025 ± 0.005 per min.

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This Item	S7446	S7571	S8409
Sigma-Aldrich S5395 Superoxide Dismutase from bovine erythrocytes	Sigma-Aldrich S7446 Superoxide Dismutase from bovine erythrocytes	Sigma-Aldrich S7571 Superoxide Dismutase from bovine erythrocytes	Sigma-Aldrich S8409 Superoxide Dismutase from bovine liver
description ≥3,000 units/mg protein, BioReagent, lyophilized powder, suitable for cell culture	description BioUltra, lyophilized powder, ≥4,500 units/mg protein, ≥97% (SDS-PAGE)	description lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret	description ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)
specific activity ≥3,000 units/mg protein	specific activity ≥4,500 units/mg protein	specific activity ≥3,000 units/mg protein	specific activity 2,000-6,000 units/mg protein (biuret)
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) inhibition assay: suitable, immunoblotting: suitable	technique(s) -
biological source bovine erythrocytes	biological source -	biological source bovine	biological source -
form lyophilized powder	form lyophilized powder	form lyophilized powder	form ammonium sulfate suspension
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
mol wt 32.5 kDa	mol wt 32.5 kDa	mol wt 32.5 kDa	mol wt 32.5 kDa

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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Questions

3 years ago

I have purchased this item recently. I'd like to test it in a cell culture medium we're developing. I was wondering what buffer I should reconstitute the protein in and whether this buffer would be appropriate for long-term frozen storage?

1 answer
1. Technical Support
  
  3 years ago
  
  The buffer recommended to reconstitute this product is 0.1 M potassium phosphate, pH 7.5. The stock solution may be stored at –20 °C. This information is available in the product datasheet.
  Please see the link below to review this, as well as additional information.
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/320/129/s5395dat.pdf
  
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