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Superoxide Dismutase from bovine liver

Name: Superoxide Dismutase from bovine liver
Brand: SIGMA
Price: 79.8 CAD

ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)

Synonym(s):

SOD, Superoxide: superoxide oxidoreductase

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Pack Size	Availability	Price
3000 units	Please contact Customer Service for Availability	$79.80
15000 units	Please contact Customer Service for Availability	$195.00
30000 units	Please contact Customer Service for Availability	$334.00

About This Item

CAS Number:

9054-89-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-943-0

MDL number:

MFCD00132404

EC Number:

1.15.1.1 (BRENDA, IUBMB)

Specific activity:

2,000-6,000 units/mg protein (biuret)

$79.80

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Properties

form

ammonium sulfate suspension

Quality Level

200

specific activity

2,000-6,000 units/mg protein (biuret)

mol wt

32.5 kDa

UniProt accession no.

P00442

storage temp.

2-8°C

Gene Information

cow ... SOD1(281495)

Description

General description

Research area: Cell Signaling

Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals, and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.

Application

Superoxide Dismutase from bovine liver has been used:

for measuring mitochondrial hydrogen peroxide production in skeletal muscle
as a positive control in Cu(II) binding stability assay
as a positive control in superoxide dismutase assay
in a study to assess the inactivation of endothelial-derived relaxing factor by oxidized lipoproteins. [1]
in a study to investigate the role of hydrogen peroxide in the cytotoxicity of the xanthine/xanthine oxidase system. [2]

Biochem/physiol Actions

Superoxide Dismutase catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It plays a critical role in the defense of cells against the toxic effects of oxygen radicals. It competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Physical form

Suspension in 3.8 M (NH₄)₂SO₄, pH 7.0

Analysis Note

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Other Notes

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀of 0.025 ± 0.005 per min.

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This Item	S8160	S7446	S7571
Sigma-Aldrich S8409 Superoxide Dismutase from bovine liver	Sigma-Aldrich S8160 Superoxide Dismutase from bovine liver	Sigma-Aldrich S7446 Superoxide Dismutase from bovine erythrocytes	Sigma-Aldrich S7571 Superoxide Dismutase from bovine erythrocytes
specific activity 2,000-6,000 units/mg protein (biuret)	specific activity ≥1500 units/mg protein	specific activity ≥4,500 units/mg protein	specific activity ≥3,000 units/mg protein
Gene Information cow ... SOD1(281495)	Gene Information cow ... SOD1(281495)	Gene Information -	Gene Information cow ... SOD1(281495), SOD2(281496)
form ammonium sulfate suspension	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt 32.5 kDa	mol wt 32.5 kDa	mol wt 32.5 kDa	mol wt 32.5 kDa
UniProt accession no. P00442	UniProt accession no. P00442	UniProt accession no. -	UniProt accession no. P00442, P41976