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S8409

Superoxide Dismutase from bovine liver

Name: Superoxide Dismutase from bovine liver
Brand: SIGMA

ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)

Sinonimo/i:

SOD, Superoxide: superoxide oxidoreductase

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Informazioni su questo articolo

Numero CAS:

9054-89-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-943-0

MDL number:

MFCD00132404

Numero CE:

1.15.1.1 (BRENDA, IUBMB)

Specific activity:

2,000-6,000 units/mg protein (biuret)

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form

ammonium sulfate suspension

Quality Level

200

specific activity

2,000-6,000 units/mg protein (biuret)

mol wt

32.5 kDa

UniProt accession no.

P00442

storage temp.

2-8°C

Gene Information

cow ... SOD1(281495)

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Questo articolo	S8160	S7571	S5395
Sigma-Aldrich S8409 Superoxide Dismutase from bovine liver	Sigma-Aldrich S8160 Superoxide Dismutase from bovine liver	Sigma-Aldrich S7571 Superoxide Dismutase from bovine erythrocytes	Sigma-Aldrich S5395 Superoxide Dismutase from bovine erythrocytes
specific activity 2,000-6,000 units/mg protein (biuret)	specific activity ≥1500 units/mg protein	specific activity ≥3,000 units/mg protein	specific activity ≥3,000 units/mg protein
Gene Information cow ... SOD1(281495)	Gene Information cow ... SOD1(281495)	Gene Information cow ... SOD1(281495), SOD2(281496)	Gene Information -
form ammonium sulfate suspension	form lyophilized powder	form lyophilized powder	form lyophilized powder
UniProt accession no. P00442	UniProt accession no. P00442	UniProt accession no. P00442, P41976	UniProt accession no. -
mol wt 32.5 kDa	mol wt 32.5 kDa	mol wt 32.5 kDa	mol wt 32.5 kDa
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

General description

Research area: Cell Signaling

Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals, and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.

Application

Superoxide Dismutase from bovine liver has been used:

for measuring mitochondrial hydrogen peroxide production in skeletal muscle
as a positive control in Cu(II) binding stability assay
as a positive control in superoxide dismutase assay
in a study to assess the inactivation of endothelial-derived relaxing factor by oxidized lipoproteins. [1]
in a study to investigate the role of hydrogen peroxide in the cytotoxicity of the xanthine/xanthine oxidase system. [2]

Biochem/physiol Actions

Superoxide Dismutase catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It plays a critical role in the defense of cells against the toxic effects of oxygen radicals. It competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Physical form

Suspension in 3.8 M (NH₄)₂SO₄, pH 7.0

Analysis Note

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Other Notes

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀of 0.025 ± 0.005 per min.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Classe di stoccaggio

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Role of hydrogen peroxide in the cytotoxicity of the xanthine/xanthine oxidase system.

E M Link et al.

The Biochemical journal, 249(2), 391-399 (1988-01-15)

1. The survival of mammalian epithelial cells exposed in vitro to the xanthine/xanthine oxidase system in phosphate-buffered saline (PBS) or serum-containing medium (SCMEM) was investigated. 2. The cytotoxic effect observed depended on the composition of the medium in which the

Effect of superoxide dismutase on myocardial infarct size in the canine heart after 6 hours of regional ischemia and reperfusion: a demonstration of myocardial salvage.

L G Chi et al.

Circulation research, 64(4), 665-675 (1989-04-01)

Available data demonstrate that oxygen free radicals and derived reactive species of oxygen are produced during myocardial ischemia as well as upon reperfusion of the ischemic tissue. The present study was designed to determine if polyethylene glycol-conjugated superoxide dismutase (PEG-SOD)

Exploiting the vulnerable active site of a copper-only superoxide dismutase to disrupt fungal pathogenesis

Robinett NG, et al.

The Journal of Biological Chemistry, jbc-RA118 (2018)

Perturbed redox signaling exacerbates a mitochondrial myopathy

Dogan SA, et al.

Cell Metabolism, 28(5), 764-775 (2018)

Inactivation of endothelial derived relaxing factor by oxidized lipoproteins.

J H Chin et al.

The Journal of clinical investigation, 89(1), 10-18 (1992-01-01)

Endothelial cell derived relaxing factor (EDRF) mediated relaxation of blood vessels is impaired in vessels exposed to lipoproteins in vitro and in arteries of hyperlipidemic humans and animals. To investigate the mechanism by which lipoproteins impair the effects of EDRF

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Articoli

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocolli

HPLC Analysis of Superoxide Dismutase on Discovery® BIO PolyMA-WAX

Separation of Superoxide dismutase

Enzymatic Assay of Superoxide Dismutase

Contenuto correlato

Data Sheet - S8409

Instructions

Numero articolo commerciale globale

SKU	GTIN
S8409-3KU	04061832731438
S8409-30KU	04061832731421
S8409-15KU	04061836960780

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