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S8633

Sphingomyelinase from Staphylococcus aureus

buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)

Synonym(s):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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Size/SKUAvailabilityPrice
25 units

Estimated to ship onJanuary 22, 2027fromMILWAUKEE

$247.00
50 units
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$418.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
MDL number:
Specific activity:
100-300 units/mg protein (Lowry)

$247.00


Estimated to ship onJanuary 22, 2027Details


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form

buffered aqueous glycerol solution

Quality Segment

specific activity

100-300 units/mg protein (Lowry)

storage temp.

2-8°C

Application

Sphingomyelinase from Staphylococcus aureus has been used to:
  • induce neurotoxicity in rat cortical cultures to study the protective effects of minocycline
  • determine the concentration of sphingomyelin from serum samples
  • enhance sphingomyelinase activity to study PARK9-mediated exosome biogenesis

Biochem/physiol Actions

Bacterial sphingomyelinase is active at neutral pH. When used in cell culture in vitro, it hydrolyzes the sphingomyelin on the outer leaflet of the plasma membrane and produces ceramide that is lipid-soluble. Sphingomyelinase is the key enzyme in the sphingomyelinase/ceramide pathway, which is implicated in the pathogenesis of several neurodegenerative disorders.
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Physical form

Solution in 50% glycerol containing 0.25 M phosphate buffer, pH 7.5

Other Notes

One unit will hydrolyze 1.0 μmol of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

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This Item
567707539202H9395
description

buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)

description

-

description

-

description

lyophilized powder, Protein ~60 % by Lowry, ≥10,000 units/mg protein

specific activity

100-300 units/mg protein (Lowry)

specific activity

≥100 units/mg protein

specific activity

-

specific activity

≥10,000 units/mg protein

form

buffered aqueous glycerol solution

form

liquid

form

lyophilized solid

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

300


Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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