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Anti-c-Myc-Peroxidase antibody, Mouse monoclonal

Name: Anti-c-Myc-Peroxidase antibody, Mouse monoclonal
Brand: SIGMA

clone 9E10, purified from hybridoma cell culture

Synonym(s):

Anti-MRTL, Anti-Myc, Anti-bHLHe39, Anti-v-myc avian myelocytomatosis viral oncogene homolog

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About This Item

NACRES:

NA.56

UNSPSC Code:

12352203

Conjugate:

peroxidase conjugate

Clone:

9E10, monoclonal

Application:

IHC

Citations:

Pricing and availability is not currently available.

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Properties

biological source

mouse

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

9E10, monoclonal

form

lyophilized powder

species reactivity

human

packaging

vial of 100 μL, vial of 500 μL

concentration

~2 mg/mL

technique(s)

immunoblotting: 1:250-1:500 using lysate of HEK-293T cells over expressing c-Myc fusion protein, immunohistochemistry: suitable

isotype

IgG1

UniProt accession no.

P01106

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYC(4609)

Description

General description

Monoclonal Anti-c-Myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with synthetic peptide of the human p62c-myc protein, conjugated to KLH. c-Myc is a proto-oncogene localized on long arm of human chromosome 8. The encoded protein contains an N-terminal transactivation domain. The human c-Myc proto-oncogene is the cellular homolog of the avian v-Myc gene found in several leukemogenic retroviruses.

Immunogen

Synthetic peptide of the human p62c-myc protein.

Application

Anti-c-Myc-Peroxidase antibody, Mouse monoclonal may be used in immunoblotting and immunohistology.

Biochem/physiol Actions

Monoclonal Anti-Calponin specifically recognizes smooth muscle Calponin.

c-Myc is a transcription factor involved in regulation of cell growth, proliferation, differentiation and apoptosis. It is implicated in DNA replication, RNA splicing or transcription. Increased expression of the gene is associated with the development of various types of human cancers.

Physical form

Supplied as lyophilized powder. After reconstitution with 0.1 mL/0.5 ml of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline

Preparation Note

Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 °C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB2108476	SAB5700727	M4439
Sigma-Aldrich SAB4200742 Anti-c-Myc-Peroxidase antibody, Mouse monoclonal	Sigma-Aldrich SAB2108476 Anti-MYC	Sigma-Aldrich SAB5700727 Anti-c-Myc antibody produced in rabbit	Sigma-Aldrich M4439 Anti-c-Myc antibody, Mouse monoclonal
description clone 9E10, purified from hybridoma cell culture	description affinity isolated antibody	description -	description clone 9E10, purified from hybridoma cell culture
species reactivity human	species reactivity guinea pig, mouse, rabbit, human, rat	species reactivity mouse, human, rat	species reactivity human
conjugate peroxidase conjugate	conjugate unconjugated	conjugate -	conjugate unconjugated
clone 9E10, monoclonal	clone polyclonal	clone polyclonal	clone 9E10, monoclonal
antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture
Gene Information human ... MYC(4609)	Gene Information human ... MYC(4609)	Gene Information human ... MYC(4609)	Gene Information human ... MYC(4609)
biological source mouse	biological source rabbit	biological source rabbit	biological source mouse

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Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H317

Precautionary Statements

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

13 - Non Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)