This product is tested for unit of activity per mL, so the volume varies and is lot-dependent. Please refer to the sample Certificate of Analysis for enzyme activity.
SAE0023
Alanine Racemase from Bacillus stearothermophilus
buffered aqueous solution, ≥10 IU/mg solid
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Synonym(s):
Alanine Racemase from Bacillus stearothermophilus
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| Size/SKU | Availability | Price |
|---|---|---|
100 units | Check Cart for Availability | $625.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥10 IU/mg solid
Biological source:
Bacillus sp. (B. stearothermophilus)
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biological source
Bacillus sp. (B. stearothermophilus)
Quality Segment
form
buffered aqueous solution
specific activity
≥10 IU/mg solid
mol wt
Mr 78 kDa (2 subunits 39 kDa each)
storage temp.
−20°C
Application
Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-14C]alanine to a racemic mixture of L/D- [14C]alanine †.
Biochem/physiol Actions
Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†
Physical form
Aqueous solution containing 10 mM potassium phosphate
Other Notes
One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.
1 of 1
This Item | |||
|---|---|---|---|
| description buffered aqueous solution, ≥10 IU/mg solid | description buffered aqueous glycerol solution, ~30 units/mg protein (Lowry) | description - | description - |
| specific activity ≥10 IU/mg solid | specific activity ~30 units/mg protein (Lowry) | specific activity ≥2.4 U/g | specific activity - |
| biological source Bacillus sp. (B. stearothermophilus) | biological source Bacillus subtilis | biological source Bacillus licheniformis | biological source Bacillus sp. (Bacillus cereus) |
| form buffered aqueous solution | form buffered aqueous glycerol solution | form liquid | form lyophilized |
| mol wt Mr 78 kDa (2 subunits 39 kDa each) | mol wt - | mol wt - | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the concentration and/or volume of this solution?
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Helpful?
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What is the amino acid sequence of the Bacillus stearothermophilus alanine racemase that you are selling (SAE0023)?
1 answer-
SAE0023 is isolated from Bacillus sp. The published sequence can be found here:
https://www.uniprot.org/uniprotkb/P10724/entryHelpful?
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