This product is not a full length protein. It is expressed in human HEK 293 cells as a C-terminally flag and histidine-tagged glycoprotein with a calculated molecular mass of 85.9 kDa (amino acids Gln18-Ser740, with a C-terminal 10-His tag). The product is suitable for use in indirect ELISA. Please see the link below to review the datasheet for product SAB4200886. Note that SAE0064 was used for coating in indirect ELISA assay. https://www.sigmaaldrich.com/US/en/product/sigma/sab4200886
SAE0064
Angiotensin Converting Enzyme-2, ACE2
Human recombinant, ≥1000000 U/mg, expressed in HEK 293 cells
Synonym(s):
ACE-related carboxypeptidase, ACE2, Angiotensin-converting enzyme homolog (ACEH), COVID-19 receptor, Coronavirus receptor, Metalloprotease MPROT15
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biological source
human
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
specific activity
≥1000000 U/mg
mol wt
~85.9 kDa by SDS-PAGE
impurities
≤1 EU/μg protein Endotoxin
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
Gene Information
human ... ACE2(59272)
General description
The gene for angiotensin converting enzyme-2 (ACE-2) is mapped to human chromosome Xp22.2. This protein is the first known human homologue of ACE. ACE2 has been identified from 5′ sequencing of a human heart failure ventricle cDNA library. It contains a signal peptide, one metalloprotease active site and a transmembrane domain. ACE-2 is a secreted and an integral membrane protein. It is expressed predominantly on the endothelium.
Application
Angiotensin Converting Enzyme-2 (ACE2) has been used in the ACE-2 inhibition assays for inhibitor selectivity studies. It has also been used for identifying potent ACE2-blocking monoclonal antibodies in an on-chip assay and in human-ACE2-blocking assay using a biolayer interferometry biosensor. This approach serves for rational vaccine designing and for the selection of robust immunotherapeutic agents against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.
Biochem/physiol Actions
The angiotensin converting enzyme-2 (ACE-2) functions as carboxydipeptidase. The central role of ACE-2, is to counter ACE activity by reducing the bioavailability of angiotensin (Ang)-II and increasing the Ang(1-7) formation. ACE-2 is a part of the renin-angiotensin system (RAS). Many researches show that Ace-2 ensures the protection of peripheral tissues and might be efficient to treat RAS-related diseases. Also, an imbalance in Ace-2/Ang-(1-7) and ACE/Ang-II axes is important for the onset of cardiovascular diseases. Severe acute respiratory syndrome (SARS) and human coronavirus (HCoV)-NL63 infection viruses has been shown to use its surface protein spike (glycoprotein) to bind to human ACE-2 receptor. The S protein is cleaved into subunits, S1 and S2 during the COVID-19 infection. S1 contains the receptor binding domain (RBD) which enables coronaviruses to bind to the peptidase domain (PD) of ACE2. Thus, ACE2 has become a high focus research target for COVID-19 infection.
Recombinant human Angiotensin Converting Enzyme-2 (ACE-2) is expressed in human HEK 293 cells as a C-terminally flag and histidine-tagged glycoprotein with a calculated molecular mass of 85.9 kDa (amino acids Gln18-Ser740, with a C-terminal 10-His tag). The DTT-reduced protein migrates as a 90-120 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein.
Physical form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
Other Notes
One unit is defined as the amount of enzyme required to cleave 1 picomole of the fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH in one minute, in 37 °C, pH 7.5.
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This Item | |||
|---|---|---|---|
| description Human recombinant, ≥1000000 U/mg, expressed in HEK 293 cells | description affinity isolated antibody | description affinity isolated antibody | description affinity isolated antibody, buffered aqueous solution |
| Gene Information human ... ACE2(59272) | Gene Information human ... ACE2(59272) | Gene Information human ... ACE2(59272) | Gene Information human ... ACE2(59272) |
| specific activity ≥1000000 U/mg | specific activity - | specific activity - | specific activity - |
| assay ≥95% (SDS-PAGE) | assay - | assay - | assay - |
| biological source human | biological source rabbit | biological source rabbit | biological source rabbit |
| recombinant expressed in HEK 293 cells | recombinant - | recombinant - | recombinant - |
| form lyophilized powder | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
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Is this (SAE0064, ACE2) a recombinant human ACE2 full-sequence protein? Is it suitable for ELISA application?
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