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SAE0071

HIV-1 GP120 protein

recombinant, expressed in HEK 293 cells

Synonym(s):

Glycoprotein 120 (gp120), Surface protein gp120

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100 μg

Available to ship TODAYfromMILWAUKEE

$652.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in HEK 293 cells
Mol wt:
calculated mol wt 55 kDa (The protein migrates as a 100-130 kDa protein on SDS-PAGE due to glycosylation)

$652.00


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recombinant

expressed in HEK 293 cells

Quality Segment

description

low endotoxin

assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

calculated mol wt 55 kDa (The protein migrates as a 100-130 kDa protein on SDS-PAGE due to glycosylation)

impurities

≤1 EU/μg protein endotoxin

UniProt accession no.

storage temp.

−20°C

General description

Recombinant human glycoprotein 120 (gp120) is expressed in human HEK 293 cells as an C-terminally his-tagged glycoprotein with a calculated molecular mass of 55 kDa (amino acids Lys33-Arg511, with a C-terminal 8-His tag). The DTT-reduced protein migrates as a 100-130 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding.

Biochem/physiol Actions

Envelope glycoprotein GP120 (or gp120) is a glycoprotein exposed on the surface of the HIV envelope. It was discovered by Tun-Hou Lee and Myron Essex of the Harvard School of Public Health in 1988. The protein′s name is derived from its apparent molecular weight of 120 kDa. The gp120 protein is essential for virus entry into cells as it plays a vital role in attachment to specific cell surface receptors. These receptors are DC-SIGN, Heparan Sulfate Proteoglycan and the CD4 receptor. The presence of gp120 is associated with higher levels of plasma IL-6, IL-10, and TNF-α, which may contribute to immune dysfunction during early HIV infection.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

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This Item
SAE0078SAE0077V5765
description

recombinant, expressed in HEK 293 cells

description

recombinant, ≥1,300 pmol/min/μg, expressed in HEK 293 cells

description

recombinant, ≥1,300 pmol/min/μg, expressed in HEK 293 cells

description

recombinant, expressed in HEK 293 cells, suitable for cell culture

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

form

lyophilized powder

form

liquid

form

liquid

form

lyophilized powder

mol wt

calculated mol wt 55 kDa (The protein migrates as a 100-130 kDa protein on SDS-PAGE due to glycosylation)

mol wt

calculated mol wt 66 kDa, observed mol wt 82 kDa by SDS-PAGE (The protein migrates as a 82 kDa protein on SDS-PAGE due to glycosylation)

mol wt

calculated mol wt 76 kDa, observed mol wt 92 kDa (The protein migrates as a 92 kDa protein on SDS-PAGE due to glycosylation)

mol wt

dimer 42 kDa (glycosylated)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

UniProt accession no.

P04578

UniProt accession no.

P14780

UniProt accession no.

P14780

UniProt accession no.

P15692


Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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