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SAE0078

MMP-9 pre-activated human

recombinant, ≥1,300 pmol/min/μg, expressed in HEK 293 cells

Synonym(s):

GELB, GELBCLG4B, Gelatinase, Gelatinase B, MANDP2, MMP-9, Matrix Metalloproteinase-10, Type IV collagenase

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Size/SKUAvailabilityPrice
50 μg

Available to ship onSeptember 02, 2026fromMILWAUKEE

$696.00

About This Item

EC Number:
3.4.24.35 (BRENDA, IUBMB)
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
liquid
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Mol wt:
calculated mol wt 66 kDa, observed mol wt 82 kDa by SDS-PAGE (The protein migrates as a 82 kDa protein on SDS-PAGE due to glycosylation)

$696.00

Available to ship onSeptember 02, 2026Details

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biological source

human

Quality Segment

200

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

form

liquid

mol wt

calculated mol wt 66 kDa, observed mol wt 82 kDa by SDS-PAGE (The protein migrates as a 82 kDa protein on SDS-PAGE due to glycosylation)

concentration

≥50 μg/mL

UniProt accession no.

P14780

application(s)

cell analysis

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MMP(4318)

General description

50 μg protein, determined by Bradford. The protein concentration is ≥50 μg/ml. for the lot-specific concentration, see Certificate of Analysis.

Biochem/physiol Actions

MMP-9 is a member of the matrix metalloproteinase (MMP) family of proteins. Proteins of the MMP family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8 (interleukin-8)-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of MMP-2 and -9, which are key effectors of extracellular matrix (ECM) remodeling. MMP-9 degrades various substrates including gelatin, collagen types IV and V, and elastin. MMP-9 is involved in a variety of autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and multiple sclerosis, and be regarded as a potential therapeutic target.
As with most MMPs, MMP-9 is secreted as an inactive pro-protein which is activated when cleaved by extracellular proteinases. MMP-9 can be cleaved (and thus activated) in vitro using 4-Aminophenylmercuric acetate (APMA), Cat. No. A9563. MMP-9 is secreted from neutrophils, macrophages, and a number of transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity.
Structurally, MMP9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline rich linker region, and a carboxyl terminal hemopexin like domain.
As with most MMPs, MMP-9 is secreted as an inactive pro-protein which is activated when cleaved by extracellular proteinases. This product was cleaved and activated in vitro using 4-Aminophenylmercuric acetate (APMA), Cat. No. A9563.

Physical form

Liquid solution, 0.22 mm filtered, containing 25 mM Tris, 10 mM CaCl2, 0.05% Brij-35

Other Notes

This product was pre-activated in vitro using 4-Aminophenylmercuric acetate (APMA). Thus, it is active and ready for use. In order to save our customers from handling hazardous materials, and for environmental saving, the highly toxic and fatal APMA was removed from the final preparation.

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This Item
M8945PF038444231
MMP-9 pre-activated human recombinant, ≥1,300 pmol/min/μg, expressed in HEK 293 cells

Sigma-Aldrich

SAE0078

MMP-9 pre-activated human

Matrix Metalloproteinase-9 human recombinant, >90% (SDS-PAGE), buffered aqueous solution

Sigma-Aldrich

M8945

Matrix Metalloproteinase-9 human

MMP-9, Proenzyme, Human, Recombinant, CHO Cells

Sigma-Aldrich

PF038

MMP-9, Proenzyme, Human, Recombinant, CHO Cells

MMP-9, Monomer, Human Neutrophil

Sigma-Aldrich

444231

MMP-9, Monomer, Human Neutrophil

description

recombinant, ≥1,300 pmol/min/μg, expressed in HEK 293 cells

description

recombinant, >90% (SDS-PAGE), buffered aqueous solution

description

-

description

-

biological source

human

biological source

-

biological source

-

biological source

-

assay

≥95% (SDS-PAGE)

assay

>90% (SDS-PAGE)

assay

>90% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

recombinant

expressed in HEK 293 cells

recombinant

-

recombinant

expressed in CHO cells

recombinant

-

concentration

≥50 μg/mL

concentration

-

concentration

-

concentration

-

form

liquid

form

buffered aqueous solution

form

liquid

form

liquid

mol wt

observed mol wt 82 kDa by SDS-PAGE (The protein migrates as a 82 kDa protein on SDS-PAGE due to glycosylation), calculated mol wt 66 kDa

mol wt

apparent mol wt ~93 kDa

mol wt

-

mol wt

-

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

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