SAE0090
Beta-galactoside alpha-2,6-sialyltransferase 1
≥300 units/mg protein, ST6GAL1 human recombinant, expressed in HEK 293 cells
Synonym(s):
Alpha 2,6-ST 1, B-cell antigen CD75, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6Gal I, Sialyltransferase 1
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
10 μg | Check Cart for Availability | $430.00 |
description
The specific activity of ST6Gal I is measured by its ability to transfer sialic acid from CMP-NANA to asialofetuin.
assay
≥95% (SDS-PAGE)
form
lyophilized powder
specific activity
≥300 units/mg protein
shipped in
ambient
storage temp.
−20°C
General description
Biochem/physiol Actions
Terminal sialylation has been shown to decrease Fcγ receptor binding and increase anti-inflammatory activity,3 as well as antibody-dependent cellular cytotoxicity in different studies by reduced binding of sialylated antibody towards FcγRIIIa.4-5
This recombinant ST6Gal I product can be used to study the mode of action of the enzyme, as well as its potential inhibitors. It can also be used as a glycoengineering tool to modify glycoproteins in vitro.
CMP-N-acetylneuraminate (CMP-sialic acid, CMP-NANA) to the β-D-galactosyl-1,4-N-acetyl-D-glucosaminyl termini on glycoproteins.
Sialic acids are distributed in a variety of glycolipids and glycoproteins. The sialic acid that is added to a galactose (Gal) can be bound either to the hydroxyl attached to carbon-3 of Gal to form an α-2,3 glycosidic linkage, or to the hydroxyl group attached to carbon-6 to form an α-2,6 glycosidic linkage. ST6Gal I generates a α-2,6 linkage of sialic acid on the non-reducing, terminal Galβ1 4GlcNAc residues of oligosaccharides and glycoconjugates.
Terminal sialylation has been shown to decrease Fcγ receptor binding and increase anti-inflammatory activity, as well as antibody-dependent cellular cytotoxicity in different studies by reduced binding of sialylated antibody towards FcγRIIIa.
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| description ≥300 units/mg protein, ST6GAL1 human recombinant, expressed in HEK 293 cells | description recombinant, expressed in E. coli BL21, ≥5 units/mg protein | description B4GALT1 human recombinant, expressed in HEK 293 cells, 2000 units/mg protein | description affinity isolated antibody |
| specific activity ≥300 units/mg protein | specific activity ≥5 units/mg protein | specific activity 2000 units/mg protein | specific activity - |
| assay ≥95% (SDS-PAGE) | assay - | assay 95% (SDS-PAGE) | assay - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form buffered aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in ambient | shipped in dry ice | shipped in ambient | shipped in wet ice |
| description The specific activity of ST6Gal I is measured by its ability to transfer sialic acid from CMP-NANA to asialofetuin. | description - | description - | description - |
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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