Key Documents

Mutanolysin from Streptomyces globisporus ATCC 21553

Name: Mutanolysin from <I>Streptomyces globisporus</I> ATCC 21553
Brand: SIGMA
Price: 252 USD
Availability: InStock

free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)

Synonym(s):

Mutanolysin

Select a Size

Change View

Size/SKU	Availability	Price
10 kU	Check Cart for Availability	$252.00

About This Item

CAS Number:

55466-22-3

UNSPSC Code:

12352202

NACRES:

NA.54

MDL number:

MFCD00131635

eCl@ss:

32160703

Specific activity:

≥4000 units/mg protein (biuret)

Biological source:

Streptomyces sp. (Streptomyces globisporus ATCC 21553)

Concentration:

≥40% protein

$252.00

Check Cart for Availability

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

Streptomyces sp. (Streptomyces globisporus ATCC 21553)

Quality Segment

200

form

lyophilized powder

specific activity

≥4000 units/mg protein (biuret)

mol wt

23 kDa, ~18.5 kDa

feature

DNA free

concentration

≥40% protein

technique(s)

nucleic acid detection: suitable

input

bacteria and yeast sample(s)

suitability

suitable for cell lysis

application(s)

life science and biopharma

shipped in

wet ice

storage temp.

−20°C

Description

Application

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Purified Mutanolysin SAE0092 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),⁽⁸⁾ and also from other bacteria that are difficult to lyse with lysozyme only.

Biochem/physiol Actions

Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. It lyses Listeria and other gram positive bacteria such as Lactobacillus and Lactococcus.

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

Features and Benefits

Suitable for difficult-to-lyse samples.

Microbial DNA free, minimizing DNA contamination from reagents in workflow.

Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.

Physical form

Lyophilized powder containing Ficoll^® and sodium succinate buffer salts

Other Notes

One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Legal Information

Ficoll is a registered trademark of Cytiva

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	SRE0007	M4782	M9901
Sigma-Aldrich SAE0092 Mutanolysin from Streptomyces globisporus ATCC 21553	Sigma-Aldrich SRE0007 Mutanolysin from Streptomyces globisporus ATCC 21553	Sigma-Aldrich M4782 Mutanolysin from Streptomyces globisporus ATCC 21553	Sigma-Aldrich M9901 Mutanolysin from Streptomyces globisporus ATCC 21553
description free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)	description Suitable for manufacturing of diagnostic kits and reagents, ≥4000 units/mg protein (biuret)	description 0.2 μm filtered, lyophilized powder, ≥4000 units/mg protein (biuret)	description lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified
technique(s) nucleic acid detection: suitable	technique(s) -	technique(s) -	technique(s) -
biological source Streptomyces sp. (Streptomyces globisporus ATCC 21553)	biological source bacterial (Streptomyces globisporus)	biological source -	biological source bacterial (Streptomyces globisporus)
specific activity ≥4000 units/mg protein (biuret)	specific activity ≥4000 units/mg protein (biuret)	specific activity ≥4000 units/mg protein (biuret)	specific activity ≥4000 units/mg protein (biuret)
application(s) life science and biopharma	application(s) diagnostic assay manufacturing	application(s) -	application(s) diagnostic assay manufacturing
suitability suitable for cell lysis	suitability suitable for cell lysis	suitability -	suitability suitable for cell lysis
concentration ≥40% protein	concentration ≥40% protein	concentration -	concentration -

Still not finding the right product?

Explore all of our products under Mutanolysin from Streptomyces globisporus ATCC 21553

Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

3 years ago

2000u/mLでもTEに溶けないのですが、対処法を教えてください

1 answer
1. Technical Support
  
  3 years ago
  
  This product should be soluble at 1 mg/mL in TE buffer.
  If you are still having issue dissolving the product, please contact our Technical Service team who can assist you with further troubleshooting and/or investigation. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Report Product Issues" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
  
  Helpful?

Reviews

No rating value