This material is lyophilized from a PBS solution, pH 7.4 . For assay purposes, the vial is reconstituted using Nuclease, Protease, and RNase-Free Water. Alternatively, the product may also be reconstituted in PBS, see product P3813. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/406/371/sae0094pis.pdf
SAE0094
SAvPhire™ Monomeric Streptavidin
High-affinity biotin binding—reduces aggregation and enables precise, reliable detection and purification of biotinylated molecules
Synonym(s):
mSA3
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| Size/SKU | Availability | Price |
|---|---|---|
1 mg | Check Cart for Availability | $426.00 |
5 mg | Please contact Customer Service for Availability | $1,230.00 |
About This Item
UNSPSC Code:
12352202
NACRES:
NA.25
Form:
lyophilized powder
Recombinant:
expressed in E. coli
$426.00
Check Cart for Availability
Product Name
SAvPhire™ Monomeric Streptavidin, recombinant, expressed in E. coli, High Affinity
recombinant
expressed in E. coli
form
lyophilized powder
shipped in
wet ice
storage temp.
−20°C
General description
Sequence contains N-terminal 6×-Histidine-tag and a C-terminal FLAG-tag
Biochem/physiol Actions
SAvPhire™ Monomeric Streptavidin is a variant of the biotin-binding streptavidin, which naturally exists as a tetramer. Many monomeric streptavidin proteins have rapid dissociation kinetics, which prevent their use in stable labeling applications. However, SAvphire™ Monomeric Streptavidin has been engineered to have a dissociation rate comparable to that of tetrameric streptavidin.
The size and monovalency of this product provide advantages over tetrameric streptavidin in applications such as functional cell surface labeling and atomic force spectroscopy. These advantages include:
This product can be used in traditional biotin-binding applications, including Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.
- Minimizing distribution or dynamics in the probing of biotinylated targets
- Reducing risk of aggregation, precipitation, and cross-linking in pre-incubation steps with biotinylated molecules
- Easier access to desired target
This product can be used in traditional biotin-binding applications, including Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.
Preparation Note
Once reconstituted, the solution can be stored at 2-8 °C for at least 1 month. For long term storage, aliquot and store at -20 °C.
This product is lyophilized from PBS, pH 7.4. Reconstitute the vial contents to a 4 mg/mL solution by adding water as follows:
The reconstituted product can be further diluted in water or PBS as needed.
- 1 mg vial: add 250 μL of water for a 4 mg/mL solution
- 5 mg vial: add 1.25 mL of water for a 4 mg/mL solution
The reconstituted product can be further diluted in water or PBS as needed.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
SAvPhire is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| description recombinant, expressed in E. coli, High Affinity | description - | description Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody | description Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| form lyophilized powder | form lyophilized | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in ambient | shipped in wet ice | shipped in wet ice |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Could you provide the optimal buffer in which this product best binds biotin?
1 answer-
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Could you provide the molar extinction coefficient for this product?
1 answer-
The extinction coefficient for the product in question is 2.8 (E0.1%).
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What is the isoelectric point (pI) of Product No. SAE0094, SAvPhire™ Monomeric Streptavidin?
1 answer-
The isoelectric point (pI) of Product No. SAE0094, SAvPhire™ Monomeric Streptavidin, is not determined by us. However, the theoretical pI is 5.9.
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