SAE0110
HRV-3C Protease, Biotin tagged
Recombinant protein, 0.8-1.2 mg/mL, aqueous solution
Synonym(s):
Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
0.5 mg | Available to ship TODAYfromMILWAUKEE | $270.00 |
About This Item
biological source
human (human Rhinovirus Type 14)
recombinant
expressed in E. coli
assay
≥90%
form
aqueous solution
specific activity
≥5000 U/mg
mol wt
22 kDa
concentration
0.8-1.2 mg/mL
technique(s)
protein purification: suitable
suitability
suitable for protein modification
application(s)
life science and biopharma
shipped in
dry ice
storage temp.
−20°C
General description
This biotinylated HRV-3C protease is intended for on-column cleavage of fusion proteins with an HRV-3C cleavage site. It specifically cleaves the protein of interest from a column-bound fusion protein, leaving the fusion domain or tag bound to the affinity column (e.g. Ni-NTA column) and eluting only the protein of interest. This method is advantageous over post-elution cleavage for several reasons:
- It eliminates most impurities normally associated with purification on Ni-chelating columns.
- It allows gentler elution conditions, with added flexibility in the elution buffer composition. This can mitigate protein aggregation and inactivation.
After cleavage, the protease can be removed with any avidin-conjugated or streptavidin-conjugated beads. This product has been enzymatically biotinylated with no effect on its proteolytic activity. It has no additional protein purification tags. The product is supplied in aqueous buffer (0.8–1.2 mg/mL) with 20 mM Trizma®-HCl, pH 8.0, 200 mM NaCl, 1 mM TCEP, and 50% (v/v) glycerol.
Preparation Note
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description Recombinant protein, 0.8-1.2 mg/mL, aqueous solution | description N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL | description - | description recombinant protein, aqueous solution |
| assay ≥90% | assay ≥90% (SDS-PAGE) | assay >95% (SDS-PAGE) | assay ≥90% |
| specific activity ≥5000 U/mg | specific activity ≥5000 U/mg | specific activity - | specific activity ≥10 U/μL |
| technique(s) protein purification: suitable | technique(s) protein purification: suitable | technique(s) - | technique(s) protein purification: suitable |
| biological source human (human Rhinovirus Type 14) | biological source human (human Rhinovirus Type 14) | biological source - | biological source microbial (Tobacco Etch virus) |
| form aqueous solution | form aqueous solution | form liquid | form liquid |
| concentration 0.8-1.2 mg/mL | concentration 0.8-1.2 mg/mL | concentration - | concentration - |
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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